Reusable long period microfiber grating for detection of DNA hybridization
A technology of long-period gratings and micro-fibers, applied in micro-structured fibers, clad fibers, optics, etc., can solve problems such as rising costs
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Embodiment 1
[0046] This example illustrates the fabrication of a long period microfiber grating (LPMFG).
[0047] Fabrication of Microfibers
[0048] use as figure 1 Single-mode fiber (SMF) is tapered using a commercially available coupler fabrication bench as shown in . A commercially available SMF (outer diameter D of 125 μm) was used and pulled to the scale of several microns. The SMF was heated and softened by a hydrogen flame whose dimension along the fiber was about 8mm. The torch is scanned along the fiber while the two translation stages holding the fiber are moved apart symmetrically. The following fabrication parameters can fabricate microfibers with diameters from hundreds of nanometers to several micrometers and effective waist lengths longer than about 30 mm: scan length: 5 to 10 mm, scan speed: 3 mm / s and pull speed: 0.17 mm / s. Since the microfiber (tapered beam waist) is tapered from the SMF in an adiabatic manner, it is automatically connected to its SMF pigtail. Th...
Embodiment 2
[0055] This example demonstrates that microfibers fabricated using the parameters of the present invention are more sensitive than other fibers.
[0056] studied the wavelength response of LPMFGs to the external refractive index and found that the wavelength response is 922 nm / RI for a diameter of 45 μm, which is much better than that of long period gratings fabricated using conventional single-mode fiber (the wavelength response of a 125 μm diameter LPG is about 200 nm / RI) is much higher. The long period microfiber grating has a diameter of about 45 μm, a pitch of about 385 μm, a period of about 20, and a notch of about 1544.5 nm when immersed in a liquid with a refractive index of about 1.33. Figure 4 A tapered fiber with a diameter of 45 μm is shown and Figure 5 The results are shown for the wavelength response of the reduced-diameter fiber LPG to the external refractive index.
Embodiment 3
[0058] This example demonstrates that LPMFG is a highly sensitive DNA sensor.
[0059] Figure 6A and 6B It was shown that when 1 μM and 0.5 nM of target DNA were hybridized with the corresponding probe DNA, the wavelength shift of the H5 avian influenza DNA sensor was 3.6 nm and 2.0 nm, respectively. exist Figure 7A and 7B Among them, when 1μM and 0.5nM target DNA were hybridized with the corresponding probe DNA, the wavelength shift of the H7 avian influenza DNA sensor was 3.7nm and 2.2nm, respectively. Therefore, the sensitivity of the sensor to 1 μM of H5 target DNA, 0.5 nM of H5 target DNA, 1 μM of H7 target DNA, and 0.5 nM of H7 target DNA is 3.60×10 -3 (nm / M), 4.00(nm / M), 3.70×10 -3 (nm / M) and 4.40 (nm / M). The results showed that the LPMFGDNA sensor was highly sensitive (1.82×10 -3 wavelength shift). Furthermore, it shows that the dynamic range can be from 0.5 nM to 1 μM and the detection limit is as low as 0.5 nm.
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Abstract
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