The invention provides a parameter calibration system of an acousto-optic tunable filter, comprising a controlling and processing computer, an optical grating monochrometer, a mercury argon gas light source, an optical path section, a precision electronic control rotary table, a high-precision radio frequency driver, a photomultiplier, a CCD light distribution detector and a precision electronic control translation guide rail; and the optical path section is mainly composed of a reflecting mirror, a semi-transparent semi-reflective lens, a Glan-Taylor prism, a collimating lens, an aperture and a focusing lens. The invention has complete calibrating system and high calibrating precision, can carry out automatic calibration on parameters like AOTF light beam incident angle, the off-axis angle theta alpha, the angular aperture delta, wavelength response function, wavelength frequency tuning relation, deflection angle beta and the like, is applicable to the index inspection of the AOTF product, the research of AOTF light spectrum and imaging characteristics, and provides technical parameters for the integration of AOTF spectrometer.