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2-deoxy-scyllo-inosose reductase

A reductase, mud crab technology, applied in the directions of oxidoreductase, enzymes, biochemical equipment and methods, etc., can solve the problems of unknown, unclear details, difficult to carry out reverse reaction, etc.

Active Publication Date: 2018-11-27
ASAHI KASEI KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since no enzyme was isolated in Patent Document 6, its details are unclear, but even if this reaction is catalyzed by a single enzyme, at least the enzyme activity is aimed at converting (-)-vibo-quercitol to The direction of 2-deoxy-scyllo-inosose is dominant, and it is reasonable to think that the substantial reverse reaction is difficult to proceed
[0013] However, the present inventors have not been aware of the fact that an enzyme capable of directly converting 2-deoxy-scyllo-inosose into (-)-vibo-quercitol as shown in the following reaction scheme has been reported so far

Method used

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Examples

Experimental program
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Embodiment

[0158] 1. Screening of (-)-vibo-quercitol assimilating bacteria

[0159] Add about 0.1 g of soil to 0.85% sterilized saline, and stir well. Thereafter, it was allowed to stand for 3 hours to allow sandstone or plant roots to settle, and 0.1 ml of the supernatant was applied to the quercetin agar medium (the composition is listed in Table 1). Cultivate at 30° C. for 1-2 days, and use LB agar medium (the composition is listed in Table 1) to carry out the isolation operation of the grown bacteria. The strain which became a single colony was inoculated on a slant medium (quercitol agar medium), cultured at 30°C, and then stored at 4°C. By implementing this experiment 63 times, 109 strains of soil bacteria were obtained.

[0160] 【Table 1】

[0161]

[0162] 2. Acquisition of microorganisms capable of converting 2-deoxy-scyllo-inosose (DOI) into (-)-vibo-quercitol

[0163] The 109 strains of soil bacteria obtained above were respectively inoculated into 2 mL of quercetin me...

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Abstract

[Problem] The purpose of the present invention is to produce (-)-vibo-quercitol with high efficiency by a simple process. Particularly, it is intended to utilize an enzyme capable of converting 2-deoxy-scyllo-inosose to (-)-vibo-quercitol directly. [Solution] A 2-deoxy-scyllo-inosose reductase which is originated from a microorganism capable of utilizing (-)-vibo-quercitol and has the properties (a) to (c): (a) the enzyme has a catalytic activity of converting 2-deoxy-scyllo-inosose to (-)-vibo-quercitol; (b) the activity of the enzyme becomes maximum at a pH value of 7.0 to 9.0; and (c) a polypeptide moiety in the enzyme has a molecular mass of about 36 kDa as measured by SDS-polyacrylamide electrophoresis.

Description

technical field [0001] The present invention relates to a novel 2-deoxy-scyllo-inosose reductase and a gene encoding the enzyme. In addition, the present invention relates to a method for producing (-)-vibo-quercitol using an enzyme capable of directly converting 2-deoxy-scyllo-inosose into (-)-vibo-quercitol. Background technique [0002] (-)-vibo-quercitol ((1R,2R,4S,5R)-cyclohexane-1,2,3,4,5-pentaol) is found in Ascariaceae plants with the following chemical structure compound of. [0003] 【Chemical 1】 [0004] [0005] So far, (-)-vibo-quercitol has been shown to have a potent glycemic effect. In addition, deoxyinositol amine (diokisinositol) and deoxyinositol amidine (diokisinositol) which can be obtained by amination of (-)-vibo-quercitol are also compounds that can be used as intermediates for the synthesis of various pharmaceuticals and pesticides ( Patent Documents 1 and 2). [0006] Furthermore, in recent years, the industrial usefulness of (-)-vibo-quercit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/09C12N1/15C12N1/19C12N1/21C12N5/10C12N9/04C12P7/02
CPCC12N9/0006C12N9/0008C12P7/18C12Y101/01018C12Y101/01329C12P7/02
Inventor 小西一诚伊藤伸哉黑川纯司
Owner ASAHI KASEI KK
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