SNP molecular marker for identifying and controlling purple character formation of Chinese cabbages and application thereof

A technology of DNA molecules and purple, which is applied in recombinant DNA technology, microbe determination/inspection, DNA/RNA fragments, etc., and can solve the problem of rare research on key genes for purple formation

Active Publication Date: 2016-04-20
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are few studies on the key ge

Method used

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  • SNP molecular marker for identifying and controlling purple character formation of Chinese cabbages and application thereof
  • SNP molecular marker for identifying and controlling purple character formation of Chinese cabbages and application thereof
  • SNP molecular marker for identifying and controlling purple character formation of Chinese cabbages and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1, the acquisition of molecular markers

[0080] In order to screen and obtain purple Chinese cabbage resources better and faster, and to promote the practice of molecular marker-assisted selection breeding, a purple Chinese cabbage material (09N-742) and a green Chinese cabbage material (09-680) were regenerated Sequencing data analysis found that there is a T / A mutation SNP site at base 31593020 of chromosome A03 ((300th nucleotide in Sequence 4 of the Sequence Listing)), and the SNP site is named A0331593020T / A position point. According to the A0331593020T / A site, design the following specific primers that can be used in KASP technology as molecular markers: upstream primer A0331593020T / A-FF, upstream primer A0331593020T / A-FV and downstream primer A0331593020T / A-R.

[0081] The above-mentioned A0331593020T / A-FF, A0331593020T / A-FV and A0331593020T / A-R primers were entrusted to UK LGC (Laboratory of the Government Chemist Government Chemist Laboratory) Limited...

Embodiment 2

[0090] Example 2, Application of A0331593020T / A site in identified purple and green materials

[0091] 1. DNA extraction

[0092] Genomic DNA of 518 kinds of cabbage materials to be tested in Table 1 were extracted by conventional CTAB method.

[0093] 518 materials are hybrid offspring BC of parents 09N-742 and 09-680 6 f 2 The specific methods are as follows: cross the purple parent material 09N-742 and the green parent material 09-680 to obtain F1; then use the green parent material 09-680 as the backcross parent for 6 generations to obtain a F1 consisting of 518 individual plants BC 6 f 2 (group.

[0094] The quality of the extracted DNA was tested by agarose electrophoresis and Nanodrop2100 respectively, and it was found that the extracted genomic DNA met the relevant quality requirements, that is, agarose electrophoresis showed a single DNA band without obvious dispersion; Nanodrop2100 detected A260 / 280 between 1.8-2.0 between (DNA samples without protein contamina...

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Abstract

The invention discloses an SNP molecular marker for identifying and controlling purple character formation of Chinese cabbages and application thereof. Application of A0331593020T/A loci comprises any one of the following applications A-F: A, application of identification or supplementary identification of colors of Chinese cabbages; B, application of breeding purple Chinese cabbage varieties; C, application of breeding Chinese cabbages; D, application of predicting colors of Chinese cabbages; E, application of preparing products used for identification or supplementary identification of colors of Chinese cabbages; and F, application of preparing products for predicting the colors of Chinese cabbages. The experiment proves that the selection accuracy rate in purple or green materials is 100 percent by utilizing the A0331593020T/A loci and corresponding primers thereof. The loci and corresponding primers thereof contribute to screening germplasm resources of the purple Chinese cabbages and providing material stock and technical support for genetic improvement of purple characters of the Chinese cabbages.

Description

technical field [0001] The invention relates to a SNP molecular marker for identifying and controlling the formation of the purple trait of cabbage in the field of biotechnology and its application, in particular to a SNP molecular marker for identifying the QTL-BrPur controlling the purple trait on the A03 chromosome of cabbage and its application. Background technique [0002] Varieties such as yellow-heart Chinese cabbage, orange-red Chinese cabbage, and purple pakchoi have bright-colored commodity quality, sweet-tasting flavor when eaten raw, and nutritional quality with high nutritional content, which are important targets for improving the quality of cabbage. At present, the varieties of Chinese cabbage with yellow heart, orange-red heart and purple Chinese cabbage have been widely promoted and planted, while the variety of purple Chinese cabbage is still blank. [0003] Studies have shown that the purple color of purple cabbage is caused by anthocyanins. Anthocyanins...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 于拴仓苏同兵张凤兰余阳俊张德双赵岫云汪维红王姣
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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