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Preparation of molecularly imprinted polypyrrole peroxide/gold nanoparticles modified electrode and its application in electrochemical recognition of cysteine ​​enantiomers

A technology for peroxidizing polypyrrole and modifying electrodes is applied in the fields of biotechnology and electrochemical research, and can solve the problems of less imprinting sites, increasing L-cysteine, and low recognition efficiency of chiral substances in molecularly imprinted chiral sensors. , to achieve the effect of simple and easy preparation method and improved recognition efficiency

Active Publication Date: 2017-12-05
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the relatively high density of the polymer, there are fewer imprinting sites, which makes the recognition efficiency of the molecularly imprinted chiral sensor not high for chiral substances. Cysteine) was combined to increase the doping amount of L-cysteine, and the molecularly imprinted polymer obtained by one-step dedoping by cyclic voltammetry had a higher recognition efficiency for cysteine ​​enantiomers than that without Molecularly imprinted polymers incorporating gold nanoparticles have significantly improved

Method used

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  • Preparation of molecularly imprinted polypyrrole peroxide/gold nanoparticles modified electrode and its application in electrochemical recognition of cysteine ​​enantiomers
  • Preparation of molecularly imprinted polypyrrole peroxide/gold nanoparticles modified electrode and its application in electrochemical recognition of cysteine ​​enantiomers
  • Preparation of molecularly imprinted polypyrrole peroxide/gold nanoparticles modified electrode and its application in electrochemical recognition of cysteine ​​enantiomers

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] a. Prepare a mixed solution including 0.12mM chloroauric acid and 0.1M potassium chloride, and reduce the chloroauric acid under different constant potential time conditions to obtain nano-gold modified electrodes.

[0025] b. Put the nano-gold modified electrode prepared in step a into the pre-prepared mixed solution including 2mM L-cysteine, 0.1M pyrrole, and 0.1M potassium chloride for 10min, in the potential range of –0.6~0.8V The method of cyclic voltammetry is used to polymerize the monomer pyrrole. After the polymerization, in the phosphate buffer solution, the cyclic voltammetry is used to dedope in the potential range of 0-1.6V, and the molecularly imprinted polypyrrole peroxide / nano gold is obtained. Modified electrodes.

[0026] c. Prepare a phosphate buffer solution containing 1mM L- / D-cysteine, insert the molecularly imprinted polypyrrole peroxide / nano-gold modified electrode prepared in step b into the electrode containing L- / D-cysteine After standing in ...

Embodiment 2

[0029] The preparation process of the molecularly imprinted polypyrrole peroxide / gold nanometer modified electrode and the recognition method for cysteine ​​are the same as those in Example 1.

[0030] In order to examine the effect of the thickness of the molecularly imprinted membrane on the recognition efficiency of L- / D-cysteine ​​in step b, cyclic voltammetry polymerization was used for 5 cycles, 10 cycles, 15 cycles, 20 cycles, and 25 cycles for the final recognition effect, the result is figure 2 As shown, the thickness of the molecularly imprinted membrane is closely related to the number of polymerization circles. Although the doping amount will increase as the thickness of the film increases, but when the number of polymerization circles exceeds 15, the too thick film will make it difficult for the template molecules to escape. Reduced recognition efficiency.

Embodiment 3

[0032] The preparation process of the molecularly imprinted polypyrrole peroxide / gold nanometer modified electrode and the recognition method for cysteine ​​are the same as those in Example 1.

[0033] In order to examine the influence of the enrichment time on the recognition efficiency of L- / D-cysteine ​​in step c, the influence of the enrichment time of 6min, 8min, 10min, 12min and 14min on the final recognition was used respectively, and the results are as follows image 3 As shown, as the enrichment time increases, the target molecules will enter the cavity of the imprinted membrane. When the enrichment time is 10 min, the recognition efficiency reaches the highest. As the enrichment time is longer than 10 min, more target molecules It will be adsorbed on the surface of the membrane and reduce the separation efficiency.

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Abstract

The invention relates to a method for preparing a molecularly-imprinted overoxidized polypyrrole and nanogold-modified electrode and applying the molecularly-imprinted overoxidized polypyrrole and nanogold-modified electrode to electrochemical recognition of a cysteine enantiomer. The method comprises the following steps of nanogold-modified electrode preparation, molecularly-imprinted overoxidized polypyrrole and nanogold-modified electrode preparation and electrochemical recognition of the cysteine enantiomer. The method for preparing the molecularly-imprinted overoxidized polypyrrole and nanogold-modified electrode has the advantages that simpliness, convenience and practicability are achieved, the preparation process is environmentally friendly and free of pollution, the imprinting locus number is increased through adsorption of nanogold to L-cysteine, and therefore the material has the higher recognition efficiency on the cysteine enantiomer compared with a molecularly-imprinted polymer-modified electrode without introducing the nanogold.

Description

technical field [0001] The invention relates to a method for preparing molecularly imprinted polypyrrole peroxide / nano-gold modified electrodes and its application in electrochemical recognition of cysteine ​​enantiomers, belonging to the fields of biotechnology and electrochemical research. Background technique [0002] Chirality is the essential attribute of organisms, and chiral selectivity is also an inevitable law in the life process. For example, in the process of a series of life activities such as ingestion, absorption, and metabolic transformation of chiral substances in organisms, chirality is often involved. Sexual selectivity, and the biological effects of chiral substances, including carcinogenicity, mutagenesis, and endocrine interference, will also exhibit chiral selectivity. Therefore, establishing a fast, accurate and sensitive method is of great significance and research value for chiral analysis. [0003] For the identification of chiral substances, there...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 孔泳顾嘉卫
Owner CHANGZHOU UNIV