Tissue culture rapid propagation method of Japanese maple orange dream

Abstract

The invention discloses a tissue culture rapid propagation method of Japanese maple orange dream. The tissue culture rapid propagation method comprises the following steps: firstly, taking annual tender stems of the orange dream, and cleaning; carrying out sterilization treatment by adopting alcohol and mercury bichloride to obtain explants; inoculating the obtained explants to a starting culture medium to be cultured until the explants are differentiated to germinate and take roots, wherein the starting culture medium is composed of 1 / 2 WPM basic culture medium and the balance of 0.1mg / L-0.5mg / L IAA, 0.02mg / L-0.08mg / L IBA, 0.1mg / L-0.5mg / L NAA, 0.02mg / L-0.08mg / L GA3, 10g / L-50g / L sucrose and 2g / L-10g / L agar; and finally, washing off the culture medium from tissue culture rooting seedlings which grow healthy and then directly transplanting the tissue culture rooting seedlings into a greenhouse for propagation. By adopting the tissue culture rapid propagation method to propagate the orange dream, a cultivation period is short, a root system is good and the survival rate is high.

Description

technical field [0001] The invention relates to the technical field of plant vegetative propagation, in particular to a tissue culture rapid propagation method of Acer palmatum orange dream. Background technique [0002] Acer palmatum (Acerpalmatum), also known as maple palmatum, belongs to the small deciduous trees or shrubs of the Aceraceae family. As an excellent ornamental colorful leaf plant, it has a long history of application in Chinese gardens, and it is more common in today's urban gardens. Orange Dream (Acerpalmatum 'Orange Dream') is one of the horticultural variants of the Acer palmatum. In spring, the new leaves are golden yellow, with orange-red edges, very beautiful. Leaves turn light green to yellow-green in summer. Foliage color turns bright yellow to orange again in autumn. The variety grows upright, compactly, and can reach a height of 3 meters. Strong growth. It can also be cultivated as a small tree. Orange represents depth, dreamlike beauty, com...

AI Technical Summary

Problems solved by technology

[0003] At present, Orange Dream is mainly propagated by sowing and cuttings, but the germination rate and survival rate are not high, which is far from meeting the domestic demand for seedlings

At the same time, the current conventional cutting propagation method of Orange Dream has a long breeding cycle, unstable rooting rate, underdeveloped root system, obvious main root, poor development of lateral roots, and poor growth performance after afforestation, which is not conducive to the large-scale production and development of Orange Dream. Develop application