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Molecular markers of type I diabetes

A diabetes, type I technology, applied in the analysis of materials, biological testing, disease diagnosis, etc., to achieve timely genetic diagnosis and improve the quality of life.

Active Publication Date: 2018-12-25
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the diagnosis of type 1 diabetes mainly relies on clinical examination and various auxiliary technologies. Although medical technology has been highly developed compared with the past, there are no reliable biomarkers in clinical practice that can predict the risk and early diagnosis of type 1 diabetes. diagnosis

Method used

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  • Molecular markers of type I diabetes
  • Molecular markers of type I diabetes
  • Molecular markers of type I diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Screening for gene markers associated with type 1 diabetes

[0046] 1. Sample collection

[0047] 10 normal blood samples and blood samples from patients with type 1 diabetes were collected, and all the above samples were obtained with the consent of the ethics committee.

[0048] 2. RNA sample preparation and quality analysis

[0049] 2.1 Preparation of RNA samples

[0050] (1) Homogenization treatment

[0051] Take blood directly, add 3 times the volume of erythrocyte lysate, mix well, place at room temperature for 10 minutes, and centrifuge at 10,000 rpm for 1 minute. Discard the supernatant thoroughly and collect the white blood cell pellet. Add 1ml Trizol per 100-200μl blood collected leukocyte pellet.

[0052] (2) layered

[0053] a. After adding TRIzol to the sample, place it at room temperature for 5 minutes to fully lyse the sample. Centrifuge at 12,000rpm at 4°C for 10min, and take the supernatant;

[0054] b. Add 200 μl of chloroform to ever...

Embodiment 2

[0076] Example 2 QPCR sequencing to verify the differential expression of the KIAA0226L gene

[0077] 1. According to the detection results of high-throughput sequencing, the KIAA0226L gene was selected for large-sample QPCR verification. According to the sample collection method in Example 1, 70 cases of blood from patients with type I diabetes and 70 cases of normal blood were selected.

[0078] 2. The RNA extraction steps are the same as in Example 1.

[0079] 3. Reverse transcription: use the reverse transcription kit of TAKARA company to operate. Specific steps are as follows:

[0080] (1) Take 2 μg of total RNA for reverse transcription, add 2 μl of Oligo(dT) and mix well; 70°C water bath; 5 min and then immediately ice bath for 2-3 min;

[0081] (2) Construct a 25 μl reaction system, including 5 μl of 5× reverse transcription buffer, 5 μl of dNTP (2.5 mM), 40 U / μl of RNasin, 200 U / μl of M-MLV, and make up to 25 μl of nuclease-free water;

[0082] (3) After 60 min in...

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Abstract

The invention discloses a molecular marker of diabetes mellitus type I. A KIAA0226L gene has up-regulated expression in blood of patients with diabetes mellitus type I, and early diagnosis can be provided for the patients with diabetes mellitus type I through detection of the expression level of the KIAA0226L gene, so that early discovery and early treatment are realized and the death rate of the patients with diabetes mellitus type I is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker of type I diabetes. Background technique [0002] Diabetes mellitus is a metabolic disorder characterized by high and chronic blood glucose (referred to as blood sugar) levels caused by absolute or relative lack of insulin. It is one of the most important non-communicable diseases currently threatening global human health. According to statistics from the Union, the number of diabetic patients in the world has reached 370 million in 2011, 80% of which are in developing countries. It is estimated that there will be nearly 550 million diabetic patients in the world by 2030. In 2011, a total of 4.6 million people died of diabetes worldwide, and the global medical cost of diabetes reached 465 billion US dollars in that year. Among them, the rapid growth of diabetes in China and other developing countries has brought a heavy burden to the social and economic developmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6893G01N2800/042
Inventor 宋宏涛肖枫
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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