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A rapid method for identifying the authenticity and purity of edible sunflower hybrid sh363

A technology of SH363 and sunflower, which is applied in the field of agricultural molecular biology, can solve the problems of being easily affected by climate and cultivation conditions, taking a long time, and untimely guidance, so as to achieve accurate and reliable detection and identification of authenticity and purity, and the measurement results Accurate and simple operation steps

Active Publication Date: 2018-08-31
三瑞农业科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are following defects in above-mentioned identification method: (1) be limited by experience, do not have completely consistent judging standard; (2) identification in sunflower growth period is also easily affected by climate and cultivation condition, causes error or misjudgment; (3) ) need to invest a lot of manpower and material resources, high cost, time-consuming, poor timeliness, the guidance of the market and production is not timely, it is difficult to avoid the loss of production
The variety has clean foliage at maturity and is resistant to downy mildew and Verticillium wilt; very low incidence of Sclerotinia

Method used

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  • A rapid method for identifying the authenticity and purity of edible sunflower hybrid sh363
  • A rapid method for identifying the authenticity and purity of edible sunflower hybrid sh363
  • A rapid method for identifying the authenticity and purity of edible sunflower hybrid sh363

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Effect test

Embodiment 1

[0071] The method for quickly identifying the authenticity and purity of the edible sunflower hybrid SH363 of the present invention comprises the following steps:

[0072] (1) Take the sunflower true leaves planted by the edible sunflower hybrid SH363 to be tested and the standard sunflower seeds A436 and R1843 as materials, and use the CTAB method to extract the genomic DNA of the above-mentioned edible sunflowers. The specific steps are as follows:

[0073] a) Take 10 mg (about 1 cm) of the edible sunflower true leaf 2 ) was placed in a 2mL centrifuge tube with grinding beads (D=0.6mm stainless steel beads), placed in liquid nitrogen for pre-freezing, and the pre-frozen centrifuge tube was placed in a grinder and ground for 30 s at 30 Hz, then Quickly transfer the centrifuge tube to stand-by in liquid nitrogen;

[0074] b) Take out the centrifuge tube in step a) and add 1000 μL of the preheated mixture of CTAB buffer and mercaptoethanol with a volume ratio of 20:1, shake an...

Embodiment 2

[0104] The method for quickly identifying the authenticity and purity of the edible sunflower hybrid SH363 of the present invention comprises the following steps:

[0105] (1) Take the sunflower true leaves planted by the edible sunflower hybrid SH363 to be tested and the standard sunflower seeds A436 and R1843 as materials, and use the CTAB method to extract the genomic DNA of each of the above-mentioned edible sunflowers. The steps are as follows:

[0106] a) Take 10 mg (about 1 cm) of the edible sunflower true leaf 2 ) was placed in a 2mL centrifuge tube with grinding beads (D=0.6mm stainless steel beads), placed in liquid nitrogen for pre-freezing, and the pre-frozen centrifuge tube was placed in a grinder and ground for 30 s at 30 Hz, then Quickly transfer the centrifuge tube to stand-by in liquid nitrogen;

[0107] b) Take out the centrifuge tube in step a) and add 1000 μL of the preheated mixture of CTAB buffer and mercaptoethanol with a volume ratio of 20:1, shake and...

Embodiment 3

[0136] The method for quickly identifying the authenticity and purity of the edible sunflower hybrid SH363 of the present invention comprises the following steps:

[0137] (1) Take the sunflower true leaves planted by the edible sunflower hybrid SH363 to be tested and the standard sunflower seeds A436 and R1843 as materials, and use the CTAB method to extract the genomic DNA of each of the above-mentioned edible sunflowers. The steps are as follows:

[0138] a) Take 10 mg (about 1 cm) of the edible sunflower true leaf 2 ) was placed in a 2mL centrifuge tube with grinding beads (D=0.6mm stainless steel beads), placed in liquid nitrogen for pre-freezing, and the pre-frozen centrifuge tube was placed in a grinder and ground for 30 s at 30 Hz, then Quickly transfer the centrifuge tube to stand-by in liquid nitrogen;

[0139] b) Take out the centrifuge tube in step a) and add 1000 μL of the preheated mixture of CTAB buffer and mercaptoethanol with a volume ratio of 20:1, shake and...

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Abstract

The present invention discloses a rapid method for identification of edible sunflower hybrid SH363 authenticity and purity, SR-366, SR-495, SR-1067 and SR-1079 are screened from a large number of SSR primers, an optimal combination suitable for PCR amplification using edible sunflower hybrid SH363 and edible sunflower hybrid SH363 parental standard sunflower seed A436 and R1843 genomic DNA as a template is obtained from the primers, by comparison with edible sunflower hybrid SH363 and edible sunflower hybrid SH363 parental standard sunflower seed A436 and R1843 electrophoretogram obtained by gel electrophoresis treatment, the edible sunflower hybrid SH363 authenticity and purity can be identified, the method is convenient, fast, and simple in operation, and can be used for rapid identification of a large number of to-be-tested edible sunflower hybrids SH363, and measurement results are accurate.

Description

technical field [0001] The invention belongs to the field of agricultural molecular biology, and in particular relates to a method for rapidly identifying the authenticity and purity of edible sunflower hybrid SH363. Background technique [0002] Sunflower (Helianthus annuus L.) is a dicotyledonous plant of the genus Helianthus in the family Compositae (Composicae). The genus Helianthus consists of 51 species and 19 subspecies, including 37 species of perennial sunflowers and 14 species of annual sunflowers. Native to southwestern North America, sunflower is the only crop that originated and domesticated in North America and is widely cultivated around the world. Sunflower is divided into oil-use sunflower and edible sunflower. The chromosome base number is 17, and there are two times, four times, and six times different levels. Among them, the cultivated species are diploid and belong to insect-borne cross-pollinated crops. Different multiple levels such as double, quadrup...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
Inventor 张永平马德甯司立平姚梅园万县贞
Owner 三瑞农业科技股份有限公司