Detection method of tomato transgenic component

Abstract

The invention discloses a detection method of a tomato transgenic component, which belongs to the field of biotechnology. The method comprises the following steps: determining a transgenic component, an endogenic standard gene and an exogenous standard gene; preparing multiple amplification primers; sampling, and mixing to obtain a mixed sample; sampling a genome of the mixed sample, and adding the exogenous standard gene to obtain mixed nucleic acid; establishing a high-flux sequencing library; performing the high-flux sequencing for the high-flux sequencing library to obtain a sequenced fragment group; obtaining the quantity of the sequencing fragments of the transgenic component in a sample to be detected, the quantity of the sequencing fragments of the endogenic standard gene and the quantity of the sequencing fragments of the exogenous standard gene; according to the quantity of the sequencing fragments of the exogenous standard gene, judging whether the experiment is successful or not, and calculating the content of the transgenic component in the sample to be detected; and according to the content of the transgenic component, judging whether the sample to be detected contains the transgenic component or not. By adopting the method, various transgenic components in the sample to be detected can be simultaneously detected quantitatively.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for detecting genetically modified components of tomato. Background technique [0002] Tomato is an important vegetable in my country. Tomato transgenic technology has matured. The planting and promotion of transgenic tomato varieties requires strict approval. Therefore, transgenic tomatoes need to be supervised. The detection of transgenic ingredients is the technical support for transgenic supervision. [0003] The existing genetically modified component detection technology mainly detects nucleic acid or protein. Among them, the main process of the nucleic acid-based detection method is: extract the nucleic acid in the sample to be tested, and use PCR (Polymerase Chain Reaction, polymerase chain reaction) method to amplify the transgene in the sample. Components, using real-time quantitative, agarose electrophoresis or chip technology to detect the presence of gene...

AI Technical Summary

Problems solved by technology

[0005] There are many kinds of genetically modified ingredients, and the existing technology can only detect one or a few of them at a time. Therefore, it is necessary to test the possible multiple genetically modified ingredients one by one before they can be determined as "non-genetically modified" products; Transformed genetically modified components, not the exogenous gene itself, therefore, for unlabeled transgenics, existing detection methods will be invalid; detection is basically qualitative detection, but quantitative detection has its practical needs, for example, adjacent A small amount of pollen from the genetically modified tomato varieties in the field has been transferred to non-transgenic varieties. During qualitative testing, non-transgenic varieties will be misjudged as genetically modified varieties and will not be approved or given administrative penalties.

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