A miniaturized cell culture device for space experiments

Abstract

The invention provides a cell culture device for miniaturized space experiments. The cell culture device can automatically control the cell culture process and perform on-line observation in space and in equivalent harsh environments. The cell culture device comprises a high-strength bearing mechanism, an environmental parameter detection and control unit, an on-line observation unit and a cell culture chip unit, wherein the high-strength bearing mechanism is used for providing a support structure and a thermal-insulation environment for the cell culture chip unit; the environmental parameter detection and control unit is used for detecting temperature, humidity and CO2 gas concentration of the cell culture environment and sending the detection information to a detection and control assembly, thereby implementing environmental parameter feedback adjustment; the cell culture chip unit is used for providing a support structure and a gas / liquid flow channel port for a microfluidic chip or other minitype culture devices; and the on-line observation unit is used for providing a window structure, which is provided with light control and suitable for optical microscope observation, for real-time observation in the cell culture process.

Description

technical field [0001] The invention belongs to the technical field of space biological experiments of a micro total analysis system, and relates to a microfluidic chip, a dynamic culture device and a method for autonomously controlling the in vitro culture and observation process of cells, in particular to a cell culture device for miniaturized space experiments. Background technique [0002] In vitro cell culture technology is the basis of cell biology research and occupies a very important position in the field of biotechnology research. Conventional cell culture uses cell culture flasks, CO 2 Devices such as incubators to cultivate cells cannot accurately reproduce the microenvironment in the body, and are not suitable for long-term online observation of cells. Since the 1990s, the development of microfluidic technology has provided new ideas and methods for cell culture. The channel size of a microfluidic chip is generally tens to hundreds of microns, which is roughly...

AI Technical Summary

Problems solved by technology

During the cultivation process, real-time morphology observation, biochemical detection, microenvironment regulation and other operations often have the risk of contamination. At the same time, it is not conducive to ensuring the repeatability and parallelism of the experiment, which brings difficulties and risks to the experiment.

Automated culture can greatly reduce these risks and difficulties. Most of the automated cell culture devices based on microchips at home and abroad are bulky, high power consumption, expensive, and limited by high processing requirements. Miniaturization, low power consumption, high reliability and other requirements

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