Qualitative and quantitative detection method for paddy microorganisms

Abstract

The invention discloses a qualitative and quantitative detection method for paddy microorganisms and belongs to the technical method of biology. The method comprises the following steps: determining target microorganism groups, target microorganisms and non-target microorganisms of a to-be-measured sample and reference microorganisms non-existing in the to-be-measured sample; designing characteristic regions of the target microorganism groups and the target microorganisms; designing multi-amplification primers of the characteristic regions; adding the reference microorganisms and exogenous nucleic acid into the to-be-measured sample, and extracting nucleic acid of the microorganisms in the to-be-measured sample; amplifying the nucleic acid of the microorganisms by use of the designed multi-amplification primers so as to obtain characteristic sequencing fragments; qualitatively and quantitatively analyzing the microorganisms in the to-be-measured sample by use of the characteristic sequencing fragments. The qualitative and quantitative detection method is free from preculture and proliferation of microorganisms, is capable of performing high-flux, high-accuracy and high-resolution detection on multiple types of known microorganisms in the to-be-measured sample in a step, and is simple and rapid in detection process and standard in process.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a qualitative and quantitative detection method for rice microorganisms. Background technique [0002] Rice microorganisms are the basis for rice disease identification and prediction, rice food safety and other applications, and qualitative and quantitative detection is very necessary. [0003] Existing rice microbial qualitative and quantitative detection technologies include morphological counting, chip detection, 16SrRNA sequencing, metagenomic sequencing and real-time quantitative PCR (Polymerase Chain Reaction, polymerase chain reaction). Morphological counting detection requires pre-cultivation of microorganisms, which takes a long time, non-culturable microorganisms cannot be detected, and only one type of microorganism can be detected at a time, the throughput is low, the sampling amount is limited when counting, and the results are rough, and it is impossible to classify th...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of inaccurate qualitative and quantitative detection of microorganisms in the prior art, the embodiment of the present invention provides a qualitative and quantitative detection method for rice microorganisms

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