Method for detecting content of N-nitrosodimethylamine in beer
A technology of dimethylnitrosamine and beer, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high specificity requirements of instruments, difficulty in popularization and application, and large solvent consumption, so as to achieve less actual consumption , Convenient promotion and application, good selectivity and accuracy
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Embodiment 1
[0034] (1) Beer extraction: take 100mL beer, ultrasonically degas it in an ultrasonic cleaner for 5 minutes, then measure 10mL into a 50mL centrifuge tube, add 100μL 2.0mg / L internal standard D6-N, N-dimethylnitrosamine standard Application solution, that is, 200ng internal standard D6-N, N-dimethylnitrite, mix well, add 3.0g sodium chloride to dissolve to saturation, add 15mL dichloromethane, vortex extract for 2min, then centrifuge at 10000rpm for 4min, discard The upper aqueous phase and the dichloromethane layer were dehydrated with anhydrous sodium sulfate, and then blow-dried with nitrogen at room temperature to less than 0.1 mL (not dry), then added 1 mL of cyclohexane, and ultrasonically mixed to obtain a solution to be purified.
[0035] (2) Purification of beer: Take a SILICA / PSA glass column (1000mg / 6mL), first wash the activation column with 3mL dichloromethane and 5mL cyclohexane in sequence, then pass all the solution through the column, first use 5mL dichlorometh...
Embodiment 2
[0042] Take beer blank sample 10mL, add 100μL 2.0mg / L N-dimethylnitrosamine use solution, obtain beer standard sample solution A, the concentration of N-dimethylnitrosamine in beer standard sample solution A is 20μg / L.
[0043] Take beer blank sample 10mL, add 25 μ L 2.0mg / L N-dimethylnitrosamine use solution, obtain beer standard sample solution B, the concentration of N-dimethylnitrosamine in beer standard sample solution B is 5 μg / L.
[0044] Take beer standard sample solution A and beer standard sample solution B respectively, and operate according to steps (1) to (2) of Example 1:
[0045] (1) Measure 10mL to 50mL centrifuge tube, add 100μL 2.0mg / L internal standard D6-N, N-dimethylnitrosamine standard application solution, that is, 200ng internal standard D6-N, N-dimethylnitrosamine , mix well, add 3.0g sodium chloride to dissolve to saturation, add 15mL dichloromethane, vortex extract for 2min, then centrifuge at 10000rpm for 4min, discard the upper aqueous phase, deh...
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