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Rapid hydrogen peroxide test paper and preparation method thereof

A technology for detecting test paper and hydrogen peroxide, which is applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators on materials, etc.

Inactive Publication Date: 2016-05-25
KUNMING BOYIN SCIANDTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the current research in the field of rapid detection test paper, most of the scientific research personnel are more concerned about the activity and stability of the horseradish peroxidase mentioned in the method, and there is no method that clearly proposes the detection of the chromogenic agent (tetramethanone) Base benzidine) in the study of the stability of rapid detection test paper, thus limiting the storage of hydrogen peroxide test paper at low temperature (2-8 ° C)

Method used

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  • Rapid hydrogen peroxide test paper and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1. Antioxidant system preparation: Weigh 1.0000g tea polyphenols and 0.2000g vitamin c, dissolve in 100mL distilled water, then add 1.5000g sodium thiosulfate, stir to dissolve and set aside;

[0028] Step 2. Enzyme protection system preparation: Weigh 2.5000g sucrose and dissolve in 100mL distilled water, then add 1.0000g polyethylene glycol and 0.8000g bovine serum albumin, heat and dissolve on an electric furnace, cool, and set aside;

[0029] Step 3. Chromogen preparation: Weigh 1.0000g of tetramethylbenzidine, add 50mL of distilled water to dissolve, then add 20mg of horseradish peroxidase to dissolve, set aside;

[0030] Step 4. Mix the prepared antioxidant system, enzyme protection system and color developer, and stir evenly to obtain the test paper soaking solution;

[0031] Step 5. Pour the soaking liquid of the test paper into the glass dish, slowly immerse the cut filter paper (pH test paper base paper, the specification is 15*30cm) into the soaking liqu...

Embodiment 2

[0036] Step 1. Antioxidant system preparation: Weigh 1.0000g tea polyphenols and 0.2000g vitamin c, dissolve in 100mL distilled water, then add 1.0000g sodium thiosulfate, stir to dissolve and set aside;

[0037] Step 2. Enzyme protection system preparation: Weigh 2.5000g sucrose and dissolve in 100mL distilled water, then add 1.0000g polyethylene glycol and 0.5000g bovine serum albumin, heat and dissolve on an electric furnace, cool, and set aside;

[0038] Step 3. Chromogen preparation: Weigh 1.0000g of tetramethylbenzidine, add 50mL of distilled water to dissolve, then add 20mg of horseradish peroxidase to dissolve, set aside;

[0039] Step 4. Mix the prepared antioxidant system, enzyme protection system and color developer, and stir evenly to obtain the test paper soaking solution;

[0040] Step 5. Pour the soaking liquid of the test paper into the glass dish, slowly immerse the cut filter paper (pH test paper base paper, the specification is 15*30cm) into the soaking liqu...

Embodiment 3

[0044] Step 1. Antioxidant system preparation: Weigh 1.0000g tea polyphenols and 0.1000g vitamin c, dissolve in 100mL distilled water, then add 2.0000g sodium thiosulfate, stir to dissolve and set aside;

[0045] Step 2. Enzyme protection system preparation: Weigh 2.5000g sucrose and dissolve in 100mL distilled water, then add 0.5000g polyethylene glycol and 1.0000g bovine serum albumin, heat and dissolve on an electric furnace, cool, and set aside;

[0046]Step 3. Chromogen preparation: Weigh 1.0000g of tetramethylbenzidine, add 50mL of distilled water to dissolve, then add 40mg of horseradish peroxidase to dissolve, set aside;

[0047] Step 4. Mix the prepared antioxidant system, enzyme protection system and color developer, and stir evenly to obtain the test paper soaking solution;

[0048] Step 5. Pour the soaking liquid of the test paper into the glass dish, slowly immerse the cut filter paper (pH test paper base paper, the specification is 15*30cm) into the soaking liqui...

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Abstract

The invention discloses rapid hydrogen peroxide test paper and a preparation method thereof. The test paper comprises a base plate and a test paper piece; one end of the base plate serves as a handheld end, the other end of the base plate serves as a test paper piece paste end, the test paper piece is pasted at the test paper piece paste end, and the test paper piece is soaked in a test paper soaking solution and then dried to obtain the test paper; the test paper soaking solution is prepared by mixing an antioxidant system, an enzyme protection system and a color developing agent, the antioxidant system is prepared by dissolving tea polyphenol, vitamin c and sodium thiosulfate into water, and the enzyme protection system is prepared by dissolving sucrose, polyethylene glycol and bovine serum albumin into water. Accordingly, groups, prone to oxidization, of tetramethyl benzidine are protected by the antioxidant system, so that the rapid hydrogen peroxide test paper can be preserved for 1 year at normal temperature.

Description

technical field [0001] The invention relates to a hydrogen peroxide rapid detection test paper and a preparation method thereof, belonging to the technical field of rapid chemical detection. Background technique [0002] Hydrogen peroxide is commonly known as hydrogen peroxide solution, which is divided into food grade and industrial grade. Industrial hydrogen peroxide contains arsenic, heavy metals and other toxic substances. Some businesses are prohibited from using high-concentration industrial hydrogen peroxide in the production process of some foods that need to be whitened, such as water-fat food, canned fruit, and noodle products, in order to improve the appearance of the product. The "National Standards of the People's Republic of China Hygienic Standards for the Use of Food Additives" clearly stipulates that in the process of food processing, the amount of hydrogen peroxide used should not exceed the limit. The United States and other countries have also formulated...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 汪生云李仁华李鹏飞邓成平余廷松
Owner KUNMING BOYIN SCIANDTECH CO LTD
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