Kit and method for extracting and purifying DNA (deoxyribonucleic acid) in untouchable specimen

Abstract

The invention provides a kit and method for effectively extracting and purifying DNA (deoxyribonucleic acid) in an untouchable specimen. The kit is composed of a digestive solution, an adsorptive solution, a washing solution 1, a washing solution 2, a washing solution 3, a magnetic bead suspension and an eluting solution. The method for extracting DNA by using the kit comprises the following steps: exfoliated cell digestion, DNA adsorption, magnetic separation, gradient washing and eluting. The kit has high extraction sensitivity, and can effectively complete the extraction of DNA in an untouchable specimen. The kit has the advantages of high impurity interference resistance and high purity, and has favorable effects on extracting DNA in the specimen containing dust, clay and other impurities. The kit is simple to operate, and can be matched with an automatic extraction apparatus.

Description

technical field [0001] The invention relates to a kit and a method capable of effectively extracting DNA from highly difficult-to-contact test materials, belonging to the technical field of biological and medical testing. Background technique [0002] DNA testing technology is an important component of current forensic testing technology. Through sequencing and analysis of DNA samples collected at the scene of the case, the use of STR (short tandem repeat sequence) typing technology can accurately identify individuals, thus providing a basis for case detection and court trials. Supported by strong evidence. Typically, forensic DNA testing includes several steps such as DNA sample collection, DNA extraction and purification, amplification and sequencing, among which DNA extraction and purification are the prerequisites for subsequent testing and play a crucial role in obtaining effective STR typing. important role. For case materials with high DNA content, such as blood spo...

AI Technical Summary

Problems solved by technology

At present, single fingerprints, secondary transfer samples and high-impurity samples are all referred to as highly difficult-to-reach samples. Conventional methods, even some conventional extraction kits, cannot effectively extract and purify DNA.

However, these difficult-to-access test materials are often a crucial source of evidence in case detection, and the inability to effectively extract DNA from them directly leads to a low DNA detection rate from high-difficulty-access test materials, which cannot meet the growing demand for case inspections.

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