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Papaya tissue culture seedling transplanting method

A technology for tissue cultured seedlings and papaya, applied in botany equipment and methods, cultivation, medium, etc., can solve the problem of reducing the survival rate of tissue cultured seedlings transplanted, affecting the growth of papaya tissue cultured seedlings, poor air permeability and water retention and other problems, to achieve the effect of easy survival and growth, improve the survival rate of transplanting, and promote the acceleration of rooting

Inactive Publication Date: 2016-06-08
广西壮族自治区农业科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the transplanting process of papaya tissue culture seedlings, due to transplanting from sterile medium to bacteria-carrying planting substrate, because the substrate is not clean, bacteria are carried to varying degrees, which can easily cause root pathogens to cause rotten roots and seedlings and reduce the growth rate of the group. The transplanting survival rate of cultivated seedlings; the traditional method needs to wash the seedlings to remove the medium before transplanting, which will cause most of the root damage and cause abnormal root system function of the plant, which will affect the growth of papaya tissue culture seedlings; the traditional cultivation medium generally uses soil, Its air permeability and water retention are poor, and it is difficult to provide appropriate water and nutrition for papaya tissue culture seedlings, thereby affecting the growth of papaya tissue culture seedlings

Method used

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  • Papaya tissue culture seedling transplanting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for transplanting papaya tissue culture seedlings, comprising the following steps:

[0025] (1) Take out the cultivated papaya tissue-cultured seedlings in clumps from the culture container, place them in the bottom and hollow containers around them, the container is a permeable basket, and each basket contains 30 clumps; the taken-out papaya tissue-cultured seedlings without removing the medium from the roots;

[0026] (2) The roots of the papaya tissue culture seedlings in the whole container are completely immersed in the disinfectant solution, and the immersion time is 5min, and then taken out; the disinfectant solution contains the following components by mass percentage: carbendazim 30%, agricultural chain 40% hymexazol and 30% hymexazol; the dilution factor of the disinfectant solution is 1000 times when used;

[0027] (3) The papaya tissue culture seedlings taken out are planted in clumps in the cultivation matrix; the cultivation matrix contains the f...

Embodiment 2

[0029] A method for transplanting papaya tissue culture seedlings, comprising the following steps:

[0030] (1) Take out the cultivated papaya tissue cultured seedlings in clumps from the culture container, place them in the bottom and hollowed out containers, the container is a water-permeable basket, and each basket contains 35 clumps; the taken out papaya tissue cultured seedlings without removing the medium from the roots;

[0031] (2) The roots of the papaya tissue culture seedlings in the whole container are completely immersed in the disinfectant solution, and the immersion time is 10min, and then taken out; the disinfectant solution contains the following components by mass percentage: 40% carbendazim, agricultural chain 20% hymexazol and 40% hymexazol; the dilution factor of the disinfectant solution is 2000 times when used;

[0032] (3) The papaya tissue culture seedlings taken out are planted in clumps in the cultivation matrix; the cultivation matrix contains the ...

Embodiment 3

[0034] A method for transplanting papaya tissue culture seedlings, comprising the following steps:

[0035] (1) Take out the cultivated papaya tissue cultured seedlings in clumps from the culture container, place them in the bottom and hollow containers around them, and the container is a permeable basket, with 25 clumps in each basket; the taken out papaya tissue cultured seedlings without removing the medium from the roots;

[0036] (2) The roots of the papaya tissue culture seedlings in the whole container are completely immersed in the disinfectant solution, the immersion time is 8min, and then taken out; the disinfectant solution contains the following components by mass percentage: carbendazim 35%, agricultural chain 30% hymexazol and 35% hymexazol; the dilution factor of the disinfectant solution is 1500 times when used;

[0037] (3) The papaya tissue culture seedlings taken out are colonized in clumps in the cultivation medium; the cultivation medium contains the foll...

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Abstract

The invention belongs to the technical field of fruit tree growing, and particularly relates to a papaya tissue culture seedling transplanting method. The method comprises the steps of 1, taking well cultured papaya tissue culture seedlings out of a culture vessel by clumps, placing the papaya tissue culture seedlings in a container with the bottom and periphery hollowed out, and determining the number of the papaya tissue culture seedlings arranged in the container according to the size of the container; 2, soaking the roots of the papaya tissue culture seedlings in the container completely in disinfectant for 5-10 min, and then taking the papaya tissue culture seedlings out; 3, planting the papaya tissue culture seedlings which are taken out in a culture medium by clumps. By the adoption of the method, the transplantation survival rate of the papaya tissue culture seedlings can be increased, and growth recovery of the papaya tissue culture seedlings is quick; the formula of the disinfectant is reasonable, the possibility of generation of damping-off pathogenic bacteria in the transplanting period of the papaya tissue culture seedlings can be reduced, and growth of roots of the transplanted papaya tissue culture seedlings is facilitated; the adopted culture medium has air permeability and a water retention function, so that survival and growth of the papaya tissue culture seedlings are facilitated.

Description

technical field [0001] The invention belongs to the technical field of fruit tree cultivation, in particular to a method for transplanting papaya tissue culture seedlings. Background technique [0002] Papaya (Caricapapaya L.), also known as papaya, milk melon, longevity fruit, is a tropical and subtropical evergreen cork large perennial herb, evergreen cork small tree, up to 8-10 meters, with latex; stems are not branched or Sometimes branched at the damaged site, with helically arranged stipules. The fruit grows on a tree and looks like a melon, hence the name papaya. The milk of papaya is the main ingredient for making loose meat powder. [0003] Papaya is distributed in tropical and subtropical regions of the world. Native to southern Mexico and adjacent central America, it is now mainly distributed in Malaysia, the Philippines, Thailand, Vietnam, Myanmar, Indonesia, India and Sri Lanka in Southeast Asia; Central and South America, the West Indies, Florida, Hawaii, an...

Claims

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Application Information

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IPC IPC(8): A01G31/00
CPCA01G31/00A01G24/00
Inventor 吴艳艳黄永才黄伟华刘洁云邓福斌吴代东牟海飞
Owner 广西壮族自治区农业科学院生物技术研究所
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