Method for interspecific hybridization young embryo culture of walnuts and juglans mandshurica maxim

A technology of hybridizing immature embryos and walnut catalpa, which is applied in the field of biological immature embryo culture, can solve the problems of uncoordinated embryo development, embryo abortion, distant kinship, etc., and achieve the effect of shortening the breeding process and improving breeding efficiency

Inactive Publication Date: 2016-06-08
POMOLOGY INST SHANXI ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

However, due to the distant relationship between the parents of interspecific hybrids, uncoordinated embryo development and embryo abortion are the main obstacles to hybridization.

Method used

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Embodiment Construction

[0023] Specific embodiments of the present invention will be described in detail below.

[0024] A method for cultivating young embryos of interspecific hybridization between walnut and Chinese catalpa, comprising the following steps:

[0025] (1) Obtaining and aseptic treatment of hybrid fruit

[0026] The high-quality walnut is used as the male parent, and the walnut catalpa is used as the female parent to carry out hybridization. After 60 days of hybridization, young fruits were taken and brought back to the laboratory for aseptic treatment. The aseptic step is as follows: the young fruit is washed with running water. Soak in 70% alcohol for 30 seconds, soak in 20% sodium hypochlorite solution for 20 minutes, then soak in 5% sodium hypochlorite solution, and put it on the ultra-clean workbench for later use.

[0027] (2) Acquisition of immature embryos

[0028] On the ultra-clean workbench, wrap the young fruit with sterile paper, clamp it with a vise, and continue to f...

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Abstract

The invention provides a method for interspecific hybridization young embryo culture of walnuts and juglans mandshurica maxim, aiming at realizing intermediate propagation of walnut hybridized seeds and shortening of a hybridized seed breeding period, and providing a new effectively seed breeding way for overcoming abortion of hybridized embryos after walnut interspecific distant hybridization. The method comprises the following specific steps: (1) obtaining hybridized fruits and carrying out sterile treatment; (2) obtaining young embryos; (3) culturing the young embryos; (4) carrying out subculture on embryo seedlings; (5) carrying out rooting induction on the embryo seedlings; (6) carrying out rooting and seedling hardening of the embryo seedlings; (7) transplanting the embryo seedlings. According to the method provided by the invention, a young embryo culture technology is combined with a conventional seed breeding procedure, and the embryo abortion in the distant hybridization can be overcome with the help of the young embryo culture technology; the method has the property of shortening a seed breeding period, can be used for rapidly selecting and breeding a walnut stock new variety with strong resistance, and is an efficient walnut stock interspecific hybridization new technology.

Description

technical field [0001] The invention relates to a technology for cultivating biological immature embryos, in particular to a method for cultivating immature embryos of interspecific hybridization of walnut and walnut catalpa. Background technique [0002] Walnut( Juglansregia L) for Juglandaceae ( Juglandaceae ) Juglans, as an economic crop, is an important tree species that has been converted from farmland to forest in recent years in my country. Our country has made remarkable achievements in the research of walnut breeding. Various local walnut germplasm resources have been fully utilized to breed a large number of excellent walnut varieties and excellent clones. However, few people in China care about the breeding research on walnut rootstocks. Walnut rootstocks have a certain influence on the grafting affinity, fruiting period, quality, tree size, adaptability and stress resistance of walnuts. They are the basis of walnut production and the only way to make my count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02
CPCA01H1/02A01H4/008
Inventor 王国平陈秋芳史华平乔永胜张拥兵樊新萍田鑫茹慧玲
Owner POMOLOGY INST SHANXI ACAD OF AGRI SCI
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