A method for separating and extracting lactic acid from fermentation broth

A technology for fermentation broth and lactic acid, applied in the biological field, can solve the problems of difficult removal of polysaccharides and macromolecular compounds, large acid-base consumption and sewage treatment, and large loss of lactic acid, etc. The effect of reducing the amount of acid and alkali used and improving the yield and quality

Active Publication Date: 2018-04-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In order to solve the problems existing in the prior art such as large loss of lactic acid, low yield, low quality of lactic acid, large consumption of acid and alkali, large amount of sewage treatment, difficult removal of polysaccharides and macromolecular compounds, etc., the present invention provides a A method for separating and extracting lactic acid from fermentation broth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take 2 L of the above fermentation broth, filter the lactic acid fermentation broth to remove solid insoluble matter, and obtain a calcium lactate solution; The pH of the solution system is 1.0. Filtrate after acidolysis to obtain a lactic acid solution after acidolysis, heat and concentrate to remove 80% of the water in the lactic acid solution, and obtain a preliminarily concentrated lactic acid solution. Heat the pre-concentrated lactic acid solution to 40°C, flow it through a carbon column at a flow rate of 2BV / h for decolorization treatment, and obtain the decolorized lactic acid serum, add 0.05 times the volume of isobutanol to the above serum, and use thin film evaporation Carry out secondary concentration and dehydration, control the vacuum degree to 90 kPa, until the lactic acid content in the lactic acid serum is 90%, and obtain the decolorized lactic acid concentrated solution. Put the above-mentioned lactic acid concentrate in an ice-water bath, and when th...

Embodiment 2

[0041] Take 2 L of the above fermentation broth, filter the lactic acid fermentation broth to remove solid insoluble matter, and obtain a calcium lactate solution; The pH of the solution system is 2.5. Filtrate after acidolysis to obtain a lactic acid solution after acidolysis, heat and concentrate to remove 80% of the water in the lactic acid solution, and obtain a preliminarily concentrated lactic acid solution. Raise the temperature of the preliminarily concentrated lactic acid solution to 65°C, add activated carbon with 1.5% mass of the lactic acid solution, and continue stirring for 25 minutes for decolorization treatment to obtain decolorized lactic acid serum, add 0.02 times the volume of isoamyl alcohol to the above serum , by using thin film evaporation for secondary concentration and dehydration, the vacuum degree is controlled at 85 kPa, until the lactic acid content in the lactic acid serum is 91%, and the decolorized lactic acid concentrate is obtained. Put the a...

Embodiment 3

[0044] Take 2 L of the above fermentation broth, filter the lactic acid fermentation broth to remove solid insoluble matter, and obtain a calcium lactate solution; The pH of the solution system is 2.0. Filtrate after acidolysis to obtain a lactic acid solution after acidolysis, heat and concentrate to remove 80% of the water in the lactic acid solution, and obtain a preliminarily concentrated lactic acid solution. Raise the temperature of the pre-concentrated lactic acid solution to 55°C, add 0.5% activated carbon of the mass of the lactic acid solution, and continue stirring for 35 minutes for decolorization to obtain the decolorized lactic acid serum, and add 0.1 times the volume of n-butanol to the above serum , by using thin-film evaporation for secondary concentration and dehydration, the vacuum degree is controlled at 85 kPa, until the lactic acid content in the lactic acid serum is 92%, and the decolorized lactic acid concentrate is obtained. Put the above-mentioned la...

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Abstract

The invention discloses a method for separating and extracting lactic acid from a fermentation broth, comprising: (1) filtering the fermentation broth, acidolysis, heating, concentrating and dehydrating to obtain a preliminarily concentrated lactic acid solution; (2) using activated carbon to decolorize the lactic acid solution, And add a small amount of high-boiling point alcohol to concentrate and dehydrate to make the lactic acid content reach more than 90%; (3) Cool down the lactic acid concentrated solution to 10-15°C, add acetate, add alcohol precipitation agent while stirring, and cool down to 4 Stop at ~10°C, filter to obtain the lactic acid filtrate and solids; (4) add ethanol to the solids to wash, filter to obtain the precipitate and lactic acid filtrate; (5) distill the lactic acid filtrate obtained in steps (3) and (4) (6) Refining the lactic acid solution obtained in step (5) by molecular distillation to obtain the lactic acid product. The invention omits the traditional desalination and deproteinization processes such as ion exchange and ultrafiltration, reduces the loss of lactic acid in the fermentation liquid, and improves the yield and quality of lactic acid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for separating microbial fermentation products, in particular to a method for separating and extracting lactic acid from fermentation liquid. Background technique [0002] As a synthetic monomer of polylactic acid and an important organic acid, lactic acid has a growing market demand and a wide range of uses, and can be used in food, medicine, medical polymer materials, chemical industry and other fields. At present, microbial fermentation is mostly used in industry to produce lactic acid. Starch (such as corn flour, rice flour, sweet potato flour, etc.), glucose and other sugars are used as raw materials, and microorganisms (such as Rhizopus oryzae, lactic acid bacteria, etc.) are inoculated to produce lactic acid through fermentation. The fermentation broth of lactic acid or lactate is separated and extracted to obtain lactic acid products. During the fermentation process, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C59/08C07C51/42
Inventor 孙启梅高大成王崇辉姚新武樊亚超
Owner CHINA PETROLEUM & CHEM CORP
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