High purity 4-hydroxyciclosporin and purification and preparation method thereof

A hydroxycyclosporine, high-purity technology, applied in the field of biopharmaceuticals, can solve the problem of high removal difficulty, and achieve the effects of shortening the process cycle, reducing the difficulty, and being difficult to recover and reuse.

Inactive Publication Date: 2016-06-08
SHIJIAZHUANG ZHONGTIAN BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main impurities in the 4-OH cyclosporine fermentation products are unconverted cyclosporine and its homologues, which are dif

Method used

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  • High purity 4-hydroxyciclosporin and purification and preparation method thereof
  • High purity 4-hydroxyciclosporin and purification and preparation method thereof
  • High purity 4-hydroxyciclosporin and purification and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0057] Example 1

[0058] 1) Take 30 L of 4-OH cyclosporine fermentation broth, containing 10.2 g of 4-OH cyclosporine, add 30 L of isopropanol to soak for 5 hours, filter the plate and frame, collect 58 L of the extract, and the titer is 173 μg / ml.

[0059] 2) Use 1LD290 resin as a decolorizing medium to decolorize the collected leaching solution at a flow rate of 2BV / h, collect the decolorizing solution, and the decolorizing solution is obviously shallower than that before the column.

[0060] 3) Use a 1LHZ816 column for adsorption of the collected decolorizing liquid, and the sample loading flow rate is 1BV / h. After the adsorption is completed, the titer of the lower column liquid is detected to be 1 μg / ml, and discarded. The adsorbed resin was washed in one step, the detergent was 45% methanol, the flow rate was 1-1.5 BV / h, and the column washing liquid had no unit after testing. Then use 80% isopropanol for analysis, the flow rate is 1BV / h, and collect the analysis solut...

Example Embodiment

[0063] Example 2

[0064] 1) Take 25 L of 4-OH cyclosporine fermentation broth, containing 8.2 g of 4-OH cyclosporine, add 20 L of ethanol to soak for 5 hours, filter the plate and frame, collect 41 L of the extract, and the titer is 189 μg / ml.

[0065] 2) Decolor the collected leaching solution with 1LRX-01T resin as a decolorizing medium at a flow rate of 2BV / h, collect the decolorizing solution, and the decolorizing solution is obviously lighter than that before the column.

[0066] 3) Use a 1LHZ816 column for adsorption of the collected decolorizing liquid, and the sample loading flow rate is 1BV / h. After the adsorption is completed, the titer of the lower column liquid is detected to be 1 μg / ml, and discarded. The adsorbed resin was washed in two steps. The detergent 1 was 45% methanol-alkali (1g / L NaOH), and the detergent 2 was 45% methanol. . Then use 82% ethanol for analysis at a flow rate of 1 BV / h, and collect the analysis solution in sections. When the analysis e...

Example Embodiment

[0069] Example 3

[0070] 1) Take 28 L of 4-OH cyclosporine fermentation broth containing 9.8 g of 4-OH cyclosporine, add 25 L of methanol to soak for 5 hours, filter the plate and frame, collect 50 L of the extract, and the titer is 185 μg / ml.

[0071] 2) Decolorize the collected extract with 1LRX-02T resin as the decolorization medium, the flow rate is 2BV / h, collect the decolorization solution, and the decolorization solution is obviously lighter than that before the column.

[0072] 3) Use a 1LHZ816 column for adsorption of the collected decolorizing liquid, and the sample loading flow rate is 1BV / h. After the adsorption is completed, the titer of the lower column liquid is detected to be 3 μg / ml, and it is discarded. The adsorbed resin was washed in two steps. The detergent 1 was 45% methanol-alkali (1g / L NaOH), and the detergent 2 was 45% methanol. . Then use 80%-85% methanol for analysis, the flow rate is 1BV / h, and collect the analysis solution in sections. When the...

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Abstract

The invention discloses high purity 4-hydroxyciclosporin and a purification and preparation method thereof. The purification and preparation method comprises the following steps: extracting 4-hydroxyciclosporin, discoloring 4-hydroxyciclosporin extract by a discoloring medium, enriching by an adsorbing medium; carrying out high pressure chromatography preparation; and performing crystallization and purification. According to the purification and preparation method, an ion exchange resin discoloring technology, a macroporous adsorption resin enriching technology, and a high pressure chromatography purification technology are adopted; in each phase, a specific technology is chosen, technologies cooperate with each other, and a good technology effect is achieved.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a method for extracting and preparing high-purity 4-hydroxycyclosporine from a 4-hydroxycyclosporin fermentation broth prepared by a biotransformation method. Background technique [0002] 4-Hydroxycyclosporine (abbreviated as 4-OH cyclosporine) is an important pharmaceutical intermediate of new anti-hepatitis C virus drugs, mainly for hepatitis C virus, referred to as hepatitis C, hepatitis C, is a hepatitis C Viral hepatitis caused by virus (HepatitisCvirus, HCV) infection. According to the statistics of the World Health Organization, the current global HCV infection rate is about 3%, it is estimated that about 180 million people are infected with HCV, and about 35,000 new cases of hepatitis C occur each year. Hepatitis C is a global epidemic, which can lead to chronic inflammation, necrosis and fibrosis of the liver, and some patients can develop liver cirrhosis and even cel...

Claims

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Application Information

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IPC IPC(8): C07K7/64C07K1/16
Inventor 魏增辉胡帅珂刘莹王乔波董国豪康志飞刘建明张志军
Owner SHIJIAZHUANG ZHONGTIAN BIOTECH
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