Cloning and application of rice gene OsPIMT2 related to low nitrogen stress and nitrogen utilization

Abstract

The invention relates to the technical field of plant molecular biology and gene engineering, and in particular relates to a rice protein (rice OsPIMT2) related to low nitrogen stress resistance as well as cloning, functional verification and application of a coding gene thereof. Full-length cDNA is cloned out by using a Smarter RACE technology to further obtain a full-length genomic sequence of OsPIMT2. Then, an over-expression and dry interference expression vector of an OsPIMT2 gene is constructed, and the agrobacterium mediated transformation of keng rice (Nipponbare) and the analysis of low nitrogen stress of a transgenic plant show that an over-expression transgenic plant of the OsPIMT2 gene, compared with a wild plant, has a lower lipid preoxidation level and a higher biomass (such as dry weight, plant height and the like). Therefore, the expression of the gene can enable the rice to use limited nitrogen to obtain and generate a higher biomass, thereby providing a certain theory and a certain basis for culturing a rice variety with a high nitrogen utilization ratio in the future.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a rice gene related to low nitrogen stress response and nitrogen utilization ( OsPIMT2 ) Isolation and cloning, functional verification and application. Background technique [0002] Nitrogen is an essential macroelement for plant growth and development. The growth of crops and food production require sufficient nitrogen, and the effects of nitrogen deficiency on growth and yield are very common. Nitrogen deficiency can lead to drastic changes in plant growth and physiology, such as growth of lateral roots, yellowing of leaves, decrease in yield, and increase of reactive oxygen species (ROS) in vivo. Therefore, nitrogen fertilizers are widely used worldwide to meet the nitrogen requirements of crops. However, although excessive use of nitrogen fertilizers will temporarily increase crop yields, the accompanying economic and environmental problems cannot be ign...

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Problems solved by technology

At present, there is no literature report in this area, but it is worthy of further exploration as a NUE-related gene

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