Hepatitis B surface antigen immune escape mutant gene detection membrane strip and PCR (polymerase chain reaction) primer

Abstract

The invention relates to a hybrid membrane strip which can be used for medically diagnosing eight common gene mutations of hepatitis B surface antigens, in particular to a hepatitis B surface antigen immune escape mutant gene detection membrane strip and a PCR (polymerase chain reaction) primer used for amplifying gene segments of to-be-detected samples. The gene detection membrane strip comprises a substrate, eight normal comparison probes and ten specific mutation detection probes, and the normal comparison probes and the specific mutation detection probes are fixed to the substrate and used for detecting nine gene mutation sites including C358A, A376T, T377G, A378C, G388G389A390 / A388A389C390, C428T, A431C, G433A and G434C. The gene detection membrane strip for detection can be used for directly making a definite diagnosis of a genotype of a to-be-detected person and has advantages of high accuracy, high specificity and the like.

Description

technical field [0001] The invention relates to a hybrid membrane strip which can be used for medical diagnosis of 8 common gene mutations of hepatitis B surface antigen, in particular to a membrane strip for detecting hepatitis B surface antigen immune escape mutation gene and PCR primers for amplifying gene fragments of samples to be tested . Background technique [0002] Hepatitis B virus (HBV) infection can cause a series of verified liver diseases, including acute and chronic hepatitis, cirrhosis and primary liver cancer. According to the World Health Organization (WHO) estimates, more than 240 million people in the world are infected with hepatitis B virus, mainly in developing countries. And as many as 786,000 people die each year from hepatitis B virus infection. [0003] Hepatitis B surface antigen is the main diagnostic marker of hepatitis B virus. Its amino acid sequence contains a highly hydrophilic region, located in the 99-169 region of amino acids, and there...

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Problems solved by technology

[0005] At present, the traditional methods in gene mutation detection mainly include mutation-specific amplification system (ARMS), single-strand conformational polymorphism analysis (PCR-SSP), DNA direct sequencing method, fluorescent quantitative PCR method, denaturing high-performance liquid chromatography These methods are not suitable for large-scale clinical promotion due to their shortcomings such as uncertainty, time-consuming and labor-intensive, expensive equipment and consumables, and difficulty in detecting multiple mutation sites of different genes at the same time. application

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