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In vitro method for the label-free determination of a cell type of a cell

A cell-type technology, applied in the direction of individual particle analysis, using optical devices, measuring devices, etc., can solve problems such as limited reproducibility, low sample throughput, and constant efforts to fix cells

Active Publication Date: 2016-06-08
SIEMENS HEALTHCARE GMBH
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Drawbacks to this approach, however, are the low sample throughput compared to using hematology analyzers and the ongoing effort to immobilize and stain cells on microscope slides
Furthermore, these stains also have only limited reproducibility and exhibit a high dependence on humidity, stain life, temperature, etc.
The downside of pure microscopy is that it quantifies cell volume compared to flow cytometry used in hematology analyzers

Method used

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  • In vitro method for the label-free determination of a cell type of a cell
  • In vitro method for the label-free determination of a cell type of a cell
  • In vitro method for the label-free determination of a cell type of a cell

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Embodiment Construction

[0036] With the help of the inventive in vitro method, the cell type of cells in a biological sample can be determined in a marker-free manner. Label-free here means that the cells do not have to be labeled with eg fluorescent dyes or radioactive particles. A biological sample may eg comprise a sample of animal or plant cells, bacterial cells and / or protozoa. It is preferably a whole blood sample, comprising eg blood cells 10, eg leukocytes, eosinophils or basophils. For clarity, different exemplary cell types are in figure 1 and figure 2 , image 3 , Figures 4a and 4b are identified by the following reference numerals: eosinophil 10', basophil 10", neutrophil 10"', lymphocyte 10"" and monocyte"" '. However, for the sake of legibility, reference numeral 10 is used for each cell type in the following description of the exemplary embodiment.

[0037] exist figure 1 In the example of , for example, the five basic white blood cells that are distinguished in the case of a s...

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Abstract

The invention relates to an in vitro method for the marker-free determination of a cell type of a cell (10) in a biological sample, wherein a microscopic device (14) detects (S1) a height profile (18) of the cell (10) with respect to a carrier plate (16). The method is characterized by the following steps performed by a cell analysis device (13): determining a cell compartment (12, 12', 12'') of the cell (10) on the basis of the detected height profile (18, S2), determining a predetermined quantitative cell feature on the basis of the determined cell compartment (12, 12', 12'', S3), and determining the cell type of the cell (10) on the basis of the determined quantitative cell feature (S3). The invention also relates to a correspondingly designed cell analysis device (13) and a corresponding microscopic device (14).

Description

technical field [0001] The present invention relates to a method for the label-free determination of the cell type of cells using a microscope device. Background technique [0002] Identifying cells and assigning them cell types is very important in cytology. The blood cell components, ie erythrocytes, platelets and leukocytes, are determined and quantified eg in blood tests. Determination of the number of white blood cells (white blood cell count, WBC) should be able to distinguish the basic populations of white blood cells for a comprehensive diagnosis. Leukocytes are differentiated by granulosa cells (neutrophils, eosinophils, and basophils) and nongranulocytes (lymphocytes, monocytes). In addition to granularity cells are also concerned with fragmentation of the nucleus (without fragmentation: mononuclear cells, i.e. lymphocytes and monocytes; polymorphonuclear cells: eosinophils, basophils and neutrophils) and cell size varies. Staining is used to differentiate gran...

Claims

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Application Information

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IPC IPC(8): G01N15/10G01N15/14G01N15/00
CPCG01N2015/1454G01N15/10G01N2015/016G01N15/1433G01N15/01G01N2015/012G01B11/0608G01N2015/1493
Inventor O·海登O·施密特
Owner SIEMENS HEALTHCARE GMBH
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