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A compound biological agent microcapsules that can improve the sensory quality of tobacco leaves

A technology of biological preparations and microcapsules, which is applied in the fields of tobacco, food science, and tobacco preparation, can solve the problems of alcoholization of tobacco leaves that have not been seen, and achieve the effects of shortening the aging time of tobacco leaves, accelerating degradation, and important application value

Active Publication Date: 2018-01-16
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there has been great progress in the research on maintaining microbial activity through microencapsulation technology, but there is no good report on the use of this technology in the alcoholization of tobacco leaves

Method used

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  • A compound biological agent microcapsules that can improve the sensory quality of tobacco leaves
  • A compound biological agent microcapsules that can improve the sensory quality of tobacco leaves
  • A compound biological agent microcapsules that can improve the sensory quality of tobacco leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The composite biological agent microcapsules provided in this example for improving the sensory quality of tobacco leaves are prepared according to the following steps.

[0047] (1) Preparation of core material:

[0048] The Bacillus subtilis bacterial solution is prepared as follows: take the Bacillus subtilis strain, LB liquid medium, 30°C, 200 rpm shaker culture and amplification, use the sterile culture medium as the blank control, and record the concentration of the bacterial solution at 600 nm The absorbance value of OD 600 , when OD 600 =6.0, as the application of bacterial agent, the count at this time shows that the number of bacteria in the bacterial liquid=7 billion / mL (specifically 7.02×10 9 cfu / mL);

[0049] The sphingomonas bacteria solution was prepared as follows: take the sphingomonas bacteria strain and the inorganic salt liquid medium, culture and amplify it on a shaker at 30°C and 200 rpm, and use the sterile culture solution as a blank control. ...

Embodiment 2

[0070] After blending HJY-0, HJY-1, and HJY-2 prepared in Example 1 with tobacco leaves in a certain proportion, the tobacco leaves were still placed in the tobacco warehouse for storage. During the storage of the tobacco leaves, samples were taken every two months. The treated tobacco leaves were evaluated. The quality changes of tobacco leaves under different treatments are introduced as follows.

[0071] The changes in the quality of tobacco leaves without adding bacterial agents (blank control) are shown in the table below:

[0072] .

[0073] When HJY-0 is used to treat tobacco leaves, add 1.0% of the mass of the tobacco leaves. The changes in the quality of the tobacco leaves after treatment are shown in the table below:

[0074] .

[0075] When HJY-1 is used to treat tobacco leaves, it is added at a rate of 2.0% of the mass of the tobacco leaves. The changes in the quality of the tobacco leaves after treatment are shown in the table below:

[0076] .

[0077]...

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Abstract

The invention belongs to the technical field of tobacco processing, and in particular relates to a compound biological agent microcapsule for improving the sensory quality of tobacco leaves. The composite biological preparation microcapsules are prepared by spray-drying uniformly mixed core material and wall material; the core material is a mixture of Bacillus subtilis bacteria liquid, Sphingomonas bacteria liquid and alkaline protease; The wall material is a mixture of corn syrup, gum arabic and maltodextrin with a mass concentration of 30-50%. The present invention encapsulates microbial preparations and enzyme preparations through microencapsulation technology to form a microenvironment conducive to the survival of microorganisms, thereby facilitating microbial preparations and biological enzymes to play a role on the surface of tobacco leaves for a long time, thereby accelerating the decomposition of macromolecular substances in tobacco leaves. Degradation, shorten the alcoholization cycle of tobacco leaves. The invention has obvious effect on improving the quality of tobacco leaves, can significantly shorten the aging time of tobacco leaves, and has relatively important application value for tobacco production enterprises.

Description

technical field [0001] The invention belongs to the technical field of tobacco processing, and in particular relates to a compound biological agent microcapsule for improving the sensory quality of tobacco leaves. Background technique [0002] Tobacco leaf aging is a key processing link to improve the sensory quality of tobacco leaves. After the tobacco leaves are harvested, threshed and re-roasted, it usually takes two to three years of aging cycle to achieve the best application quality. Improving the intrinsic quality of tobacco leaves and improving the industrial usability of tobacco through alcoholization has always been one of the hotspots in tobacco research. [0003] The essence of the alcoholization process of tobacco leaves is a synergistic process of various complex chemical degradation transformations, biological enzyme-catalyzed transformations, and microbial-promoted transformations. ) is degraded to form small molecular aroma compounds or aroma precursors, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B15/20A24B3/12
CPCA24B3/12A24B15/20
Inventor 张展杨宗灿彭玉富周浩郝辉董艳娟高明奇刘向真聂聪马宇平
Owner CHINA TOBACCO HENAN IND
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