A kind of separation and detection method of bisphenol A
A detection method and bisphenol technology, applied in the field of organic chemistry and analytical chemistry, can solve the problems of incomplete elution of template molecules, low BPA adsorption and adsorption capacity, etc., and achieve the effect of increasing adsorption capacity and specific recognition function
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[0028] A method for separating and detecting bisphenol A disclosed by the invention comprises the following steps:
[0029] (1) Sample treatment: add acetonitrile to the sample, shake and mix well, centrifuge, and take the supernatant as the sample solution to be tested.
[0030] (2) Add the magnetic molecularly imprinted polymer into the liquid to be tested, shake for 20-120 min, and magnetically separate the magnetically imprinted polymer.
[0031] (3) Eluting the bisphenol A in the magnetic molecularly imprinted polymer with a certain volume of elution solvent, and collecting the eluate.
[0032] (4) The concentration of bisphenol A in the eluate was determined by HPLC / UV.
[0033] In this embodiment, pure milk samples are taken as an example. In a 15 mL centrifuge tube, add 5 mL of pure milk followed by 5 mL of acetonitrile. After shaking for 1-2 minutes, the mixture was centrifuged at a speed of 4000 r / min for 15-30 minutes, and the supernatant was taken as the liquid ...
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