A method for rapid propagation of medicinal plant Lycium barbarum black fruit in desert area

Abstract

The invention discloses a quick propagation method for medicinal plant lycium ruthenicum in deserts. The quick propagation method comprises the following step 1, culturing of sterile seedlings (MS (Murashige) culture medium); 2, inducing of curative tissues (MS + 0.3mg / L of 6-benzylamino adenine + 1.0mg / L of 2,4-dichlorphenoxyacetic acid); 3, multiplication culture of multiple shoots (MS + 0.3mg / L of 6-benzylamino adenine + 0.3mg / L of kinetin + 0.5mg / L of naphthylacetic acid); 4, rooting culturing (1 / 2 of MS + 0.5mg / L of naphthylacetic acid + 0.5mg / L of MgSO4), wherein the culturing conditions of each step are the same, the temperature is 24+ / -2 DEG C, the light intensity is 1500-2000lx, and the light duration is 14h / d. The quick propagation method has the beneficial effects that by adopting the tissue culturing method, under the condition of artificial control, the quick propagation of lycium ruthenicum seedlings is realized, and the duration from inducing of curative tissues to the seedling rooting is only 55d; compared with the traditional sexual propagation, the segregation of character of the plant in the growth process is avoided, the propagation speed is high, the cost is low, the survival rate is high, the seedling growing speed is high, and the like.

Description

technical field [0001] The invention relates to a method for propagating medicinal plants, in particular to a method for rapidly propagating the medicinal plant Lycium barbarum black fruit in desert areas, and belongs to the technical field of plant propagation. Background technique [0002] Lycium ruthencium is mainly distributed in the Hexi Corridor and the Heihe River Basin in Gansu Province, and is a typical desert plant. It has strong adaptability to environmental conditions, and is resistant to drought, cold, barrenness, and salinity. In recent years, it has been widely promoted and planted because its fruit can be used as medicine to enhance immunity, delay aging, prevent cancer, and improve sleep. However, there are generally problems such as low survival rate and slow reproduction speed. [0003] At present, domestic and foreign research on Lycium barbarum black fruit mainly focuses on pigment extraction, pharmacological research, physiological mechanism of salt to...

AI Technical Summary

Problems solved by technology

In recent years, it has been widely promoted and planted because its fruit can be used as medicine to enhance immunity, delay aging, prevent cancer, improve sleep, etc., but there are common problems such as low survival rate and slow reproduction speed.

[0003] At present, domestic and foreign research on Lycium barbarum is mainly focused on pigment extraction, pharmacological research, physiological mechanism of salt tolerance and seedling breeding technology, while no research on tissue culture system has been reported.

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