Trichoderma konii tg-72 and its application in biological control of Aspergillus flavus
A technology of Trichoderma konii, TG-72, applied in the direction of microorganism-based methods, chemicals for biological control, applications, etc., can solve the problems of easy pollution of the environment, high cost, chemical drug resistance and even drug resistance, etc. question
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Embodiment 1
[0048] Embodiment 1: The solid fermentation culture of Trichoderma TG-72 bacterial strain, the following are weight percentages.
[0049] The Trichoderma TG-72 strain involved in the present invention has a preservation number of CCTCC NO: M 2016015, and its classification name is Trichoderoma koningii TG-72. It is isolated from the root system of peanuts in Hezhou, Guangxi. Through indoor antagonism experiments, in vivo antivirus experiments and field antivirus experiments, it is found that the strain has strong antibacterial properties, good and stable antibacterial and detoxification effects, easy to cultivate, and has no pollution. environment safety.
[0050] The solid fermentation culture and preparation of Trichoderma TG-72 bacterial strain are as follows:
[0051] (1) Incline strains: inoculate the spores or hyphae of Trichoderma TG-72 strain in PDA medium, and culture at 25-28°C for 5 days.
[0052] (2) Eggplant bottle culture: inoculate the Trichoderma strain in th...
Embodiment 2
[0057] Determination of Inhibition Rate of Metabolites of Trichoderma TG-72 Strain
[0058] Preparation of Metabolites of Biocontrol Bacteria
[0059] After the biocontrol bacterium TG-72 is activated on the PDA slant, take a ring in the PD culture medium, 100mL / 300mL liquid volume, 30°C, 160r / min shaking culture for 72h, and prepare the following treatment liquid: supernatant: The culture solution was centrifuged at 12,000 r / min for 15 minutes, and the supernatant was taken, filtered through a 0.22 μm bacterial filter to obtain a sterile supernatant.
[0060] Bacterial suspension: Centrifuge the culture solution at 12000r / min for 15min, discard the supernatant, wash with sterile water for 3 times and then centrifuge, add sterile water.
[0061] Crude protein extract: Centrifuge the culture solution at 12000r / min at 4°C for 15min to discard the precipitate, add solid ammonium sulfate to the supernatant to 70% saturation, let stand overnight at 4°C, centrifuge at 10000r / min at...
Embodiment 3
[0069] Root Colonization Experiment of Trichoderma TG-72 Strain
[0070] Peanut 8252 was germinated indoors, and germinated seeds with consistent growth were selected for sowing, 10 seeds per pot, and repeated five times. After the two cotyledons of the peanut seedlings are flattened, the inoculation begins. The spore suspension concentration of Trichoderma TG-72 strain was 2×10 6 / mL, the spore suspension was injected into the rhizosphere soil with a syringe, 15mL per hole, and clear water was used as a control. After 4 weeks, TSM medium was used to detect the Trichoderma TG-72 strain in the rhizosphere soil of potato plants, which was used as an indicator of the rhizosphere colonization ability of Trichoderma fungus. The rhizosphere soil of peanut plants not inoculated with Trichoderma was used as a control when counting. The results are shown in Table 6, which shows that no matter the bacterial strain TG-72 is inoculated alone or mixed with Aspergillus flavus, the surviv...
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