Triton X-100 enhanced biochar immobilized microbial material and its preparation method and application
A technology for immobilizing microorganisms and biochar, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of low binding force between carriers and microorganisms, unstable effects, etc., and achieve the promotion of biochar adsorption of organic pollution substances, improving practicability, and improving removal efficiency
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Embodiment 1
[0023] Pseudomonas aeruginosa JXQ was cultured in an inorganic salt medium containing 3.5mg / L acenaphthene at 30°C, centrifuged after 24 hours, and resuspended. Adjust the bacterial suspension with inorganic salt medium to obtain OD 600 100mL of 0.1 bacterial solution. Add 0.3 mL of 10 g / L Triton X-100 solution (Triton X-100 concentration is 30 mg / L) to the above bacteria solution. Add 1 g of biochar to the 100 mL bacterial solution containing Triton X-100 (mass ratio 1:100), stir at 30°C for 24 hours, and filter to obtain biochar-immobilized microbial materials.
[0024] Put 2.5 g of the prepared biochar-immobilized microbial material into 1 L of organic wastewater containing 3.5 mg / L acenaphthene and mix and stir for 4 hours, and the removal rate of acenaphthene can reach 87.4%. As a control, the removal rate of acenaphthene was 62.0% under the same conditions for biochar-immobilized microbial materials prepared without Triton X-100 enhanced conditions. It can be seen tha...
Embodiment 2
[0026] Pseudomonas aeruginosa JXQ was cultured in an inorganic salt medium containing 3.5mg / L acenaphthene at 30°C, centrifuged after 24 hours, and resuspended. Adjust the bacterial suspension with inorganic salt medium to obtain OD 600 0.2 bacteria liquid 100mL. Add 0.1 mL of 10 g / L Triton X-100 solution (Triton X-100 concentration is 10 mg / L) to the above bacteria solution. Add 1 g of biochar to 100 mL of the bacterial solution containing Triton X-100 (mass ratio 1:100), stir at 30°C for 12 hours, and filter to obtain biochar-immobilized microbial materials.
[0027] Put 2.5 g of the prepared biochar-immobilized microbial material into 1 L of organic wastewater containing 3.0 mg / L acenaphthene and mix and stir for 4 hours, and the removal rate of acenaphthene can reach 77.4%. As a control, the removal rate of acenaphthene was 70.6% under the same conditions for biochar-immobilized microbial materials prepared without Triton X-100 enhanced conditions. After strengthening w...
Embodiment 3
[0029] Pseudomonas aeruginosa JXQ was cultured in an inorganic salt medium containing 3.5mg / L acenaphthene at 30°C, centrifuged after 24 hours, and resuspended. Adjust the bacterial suspension with inorganic salt medium to obtain OD 600 0.2 bacteria liquid 100mL. Add 0.1 mL of 10 g / L Triton X-100 solution (Triton X-100 concentration is 10 mg / L) to the above bacteria solution. Add 1 g of biochar to the 100 mL bacterial solution containing Triton X-100 (mass ratio 1:100), stir at 30°C for 24 hours, and filter to obtain biochar-immobilized microbial materials.
[0030] Put 2.5 g of the prepared biochar-immobilized microbial material into 1 L of organic wastewater containing 3.5 mg / L acenaphthene and mix and stir for 4 hours, and the removal rate of acenaphthene can reach 87.7%. As a control, the removal rate of acenaphthene was 77.3% under the same conditions for biochar-immobilized microbial materials prepared without Triton X-100 enhanced conditions. It can be seen that afte...
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