A molecular marker for identifying sesame nuclear male sterile line and its identification method

Abstract

The invention provides a molecular marker for identifying sesame cell nuclear male sterile lines and an identification method thereof, wherein the molecular marker is to use Primer Premier 5.0 software to design primers InDel70F and InDel70R, and use the primers to pair recessive homozygous sterile lines When amplifying, the length of the amplified fragment is 140bp; when amplifying the dominant homozygous fertile strain, the amplified fragment length is 130bp; when amplifying the dominant heterozygous fertile strain, the amplified fragment is two, of which One is 140bp in length and the other is 130bp in length. This molecular marker can be used for assisted breeding of sesame male sterility, shortening the transfer cycle, improving the transfer efficiency, and eliminating the need for self-identification in each generation during the transfer process. The cumbersome procedure of its sterility transforms traditional phenotype selection into genotype selection, improving the accuracy and scientificity of selection. At the same time, using the molecular marker to identify the male sterile line of sesame at the seedling stage can not only ensure the purity of sesame seed production, but also save labor, reduce cost and improve work efficiency.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker for identifying sesame cell nuclear male sterile lines and an identification method thereof. Background technique [0002] Sesamum (Sesamum indicum L.) belongs to the family Flaxaceae, and is an important high-quality oil crop and characteristic agricultural product in my country, and it is also an important agricultural product for exporting foreign exchange. In recent years, the contradiction between the supply and demand of edible oil in our country is very prominent. Taking various measures to continuously increase the output of sesame and improve the production efficiency of sesame is a major topic of sesame research. The problem in sesame production is that the yield per unit area is low and unstable. Sesame varieties are very sensitive to environmental variability, including lack of tolerance to disease, waterlogging, and response to changes in light and te...

AI Technical Summary

Problems solved by technology

The problem in sesame production is that the yield per unit area is low and unstable

At present, sesame uses the male sterile two-line method for hybrid seed production. The two-line seed production technology used in production is not perfect, and only 50% of the production population is sterile. During the seed production process, it is necessary to remove the anthers according to the characteristics of the anthers during the flowering stage. Fertile plants in female parent row

This is time-consuming and laborious, increases the cost of seed production, and the purity of the seeds cannot be guaranteed, which undoubtedly limits the application of sesame hybrids to a certain extent, so the early identification of sesame male sterile lines is particularly important.

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