The primers and cloning method for cloning the full-length genome sequence of sweet potato feather mottle virus o strain and sweet potato virus c

A feathery mottle virus and genome sequence technology, applied in the field of bioengineering, can solve the problems of low content, difficult to obtain the full-length genome sequence of the virus, and no full-length genome sequence of the isolates yet.
CN105695456BActive Publication Date: 2017-08-04INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
Publication Date
2017-08-04

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Abstract

The invention discloses primers for cloning SPFMV (sweet potato feathery mottle virus) O strain and full length genome sequence of SPVC (sweet potato virus C). The primers are respectively a primer for cloning the SPFMV O strain, a primer for cloning the full length genome sequence of SPVC, and the primer for simultaneously cloning the SPFMV O strain and the full length genome sequence of SPVC. The SPFMV and the SPVC generally infect sweet potatoes in a mixed way, the separation and the purification are very difficult; the content of the virus in the sweet potatoes is low; the full length genome sequence is difficult to obtain by a conventional virus purification cloning method. The principle that SPCSV can promote the content rise of the SPFMV and the SPVC in the sweet potato plants is used; the sweet potato plants simultaneously infected with SPCSV, SPFMV-O and SPVC viruses are used as materials; the total RNA (ribonucleic acid) of the virus-infecting sweet potato plant leaves is extracted; the virus full length genome sequence cloning is performed; through a great amount of PCR (polymerase chain reaction) amplification and sequence testing, the genome full length sequence of SPVC and SPFMV is obtained through comparison and splicing.
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Description

technical field

[0001] The invention relates to primers and a cloning method for cloning the full-length genome sequence of sweet potato feather mottle virus O strain, primers and a cloning method for cloning the full-length genome sequence of sweet potato virus C, and simultaneous cloning of sweet potato feather mottle virus O strain and sweet potato virus C A primer and a cloning method for a full-length genome sequence belong to the technical field of bioengineering. Background technique

[0002] Sweet potato feathery mottle virus (Sweet potato feathery mottle virus, SPFMV) and sweet potato virus C (Sweet potato virus C, SPVC) are the main viruses that harm sweet potatoes, and both viruses belong to the family Potyviridae (Potyviridae). Potyvirus) members, often mixed infecting sweet potatoes in the field, and can co-grow with sweet potato chlorotic stunt virus (SPCSV) to cause the occurrence of sweet potato compound virus disease SPVD (sweet potato virus disease, SPVD), ...

Claims

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