Method for continuously separating synthetic pigments in multiple modes with pCEC (pressurized capillary electrochromatography)

A technology of capillary electrochromatography and capillary separation, which is applied in the field of analytical chemistry, can solve the problems of few studies on multi-mode continuous separation and no reports on multi-mode continuous separation, and achieve the effect of improving the separation effect

Active Publication Date: 2016-06-22
FUZHOU UNIV
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Problems solved by technology

At present, pCEC research mainly focuses on the preparation of monolithic polycapillary columns and the study of stationary phase structures. The monolithic columns involved are mainly single separation modes of reversed-phase, hydrophilic or mixed interaction. There are few studies on multi-mode continuous separation. Multimodal Sequential Separation Studies of Piezoelectrophoresis and Piezoelectric Chromatography Haven't Been Reported

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  • Method for continuously separating synthetic pigments in multiple modes with pCEC (pressurized capillary electrochromatography)

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Embodiment 1

[0025] A pressurized capillary electrochromatographic multi-mode continuous separation method for synthetic pigments is to use electrophoresis-electrochromatography multi-mode continuous separation technology to continuously form a multi-mode separation with a quartz capillary with a bare inner wall and a capillary chromatographic column with a chromatographic filler. Capillary column analysis, the specific method is as follows:

[0026] 1) Select a multi-mode capillary separation column with an effective column length of 15cm and an inner diameter of 100μm, of which the length of the quartz capillary part is 7.0cm, and the length of the capillary column part with packing is 8.0cm, and the packing is octadecyl bonded Silica gel particles, the particle size is 3μm;

[0027] 2) Use acetonitrile:tetrabutylammonium bisulfate (3.0mmol / L, pH4.0) = 30:70 (v / v) mixed solution as mobile phase, degassing by ultrasonic;

[0028] 3) In the way of isocratic elution, adjust the pressure of...

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Abstract

The invention discloses a method for continuously separating synthetic pigments in multiple modes with pCEC (pressurized capillary electrochromatography). According to the method, a multi-mode capillary separating column is adopted, and a high-voltage electric field and liquid-phase high voltage are applied to the separating column simultaneously, so that the synthetic pigments are firstly subjected to first-dimension pressurized capillary electrophoresis separation in a hollow capillary area with the inner surface exposed and are continuously transferred to a capillary chromatographic column area filled with chromatography filler for second-dimension pCEC separation, and electrophoresis-electrochromatography multimode continuous separation of multiple components on the same separation column is realized; the multimode capillary separation column is seamlessly and continuously constituted by a section of quartz capillary with the inner wall exposed and a section of capillary chromatographic column. Multiple functions of multimode continuous chromatography, pressurized electrophoresis, pCEC separation and the like are comprehensively applied, the procedure is continuous, and the separation efficiency is high.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a method for continuously separating and synthesizing pigments in multiple modes by pressurized capillary electrochromatography. Background technique [0002] Synthetic pigment, bright color, strong tinting strength, cheap price. Studies have pointed out that synthetic pigments cannot provide nutrition to the human body, and some synthetic pigments even endanger human health. Illegal addition of excessive synthetic pigments to food is one of the main causes of food safety problems in recent years. [0003] There are many kinds of synthetic pigments with similar structures. At present, high-performance liquid chromatography is the national standard method for detecting synthetic pigments in food, and it is also one of the most commonly used methods for detecting synthetic pigments. Gradient elution mode is required to detect and separate synthetic pigments in food, and the oper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N30/02
CPCG01N27/447G01N30/02
Inventor 林旭聪纪静静谢增鸿
Owner FUZHOU UNIV
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