Method for detecting content of cyanide in milk and milk powder

A detection method and cyanide technology, which are applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as interference in cyanide determination, no reports on cyanide detection methods, etc., and achieve good stratification, accurate and reliable determination, and low detection. out-of-limit effect

Active Publication Date: 2016-06-22
INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the large amount of protein, fat and other substances in milk and milk powder, from pretreatment to instrumental analysis, it has seriously interfered with the determination of cyani

Method used

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  • Method for detecting content of cyanide in milk and milk powder
  • Method for detecting content of cyanide in milk and milk powder
  • Method for detecting content of cyanide in milk and milk powder

Examples

Experimental program
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Effect test

Embodiment 1

[0063] Example 1: Determination of cyanide content in commercially available milk and milk powder.

[0064] (1) Sample collection and preparation:

[0065] Buy 10 different brands or different types of milk (including breakfast milk, high-calcium milk, pure milk, etc.) and 10 milk powders (whole milk powder, skimmed milk powder, infant milk powder, etc.) from the supermarket. Milk powder samples were diluted with deionized water at a ratio of 1:10 (m / v), mixed, and then processed and tested.

[0066] (2) Sample pretreatment:

[0067] Take 2.0mL of the above milk or milk powder dilution, add an appropriate amount of 0.1mol / L potassium hydroxide solution to adjust its pH to about 10, add 400μL of 0.05mol / L octadecyltrimethylammonium bromide solution and 2.0mL Pentafluorobenzyl bromide-dichloromethane (0.4%, v / v) solution, vortexed on a vortex mixer for 2min, then derivatized in a water bath at 30°C for 30min, and then derivatized at 10000r / min at 4°C Centrifuge at a rotationa...

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Abstract

The invention relates to a gas chromatography-serial four-rod mass spectrometric detection method for detecting the content of cyanide in milk and milk powder. A sample is placed under an alkaline condition; octadecyl trimethyl ammonium bromide is taken as a phase transfer catalyst; Pentafluorobenzyl Bromide (PFB-Br) is taken as a derivation reagent; the cyanide ion (CN-) is derived into a volatile derivative product Pentafluorobenzyl Bromide (PFB-Br); and gas chromatography-serial four-rod mass spectrometric (GC-MS/MS) detection is adopted and a standard curve method is adopted for quantifying, thereby acquiring the content of cyanide in the sample. The method provided by the invention is adopted for detecting the content of cyanide in milk and milk powder and has the advantages of simple and convenient operation and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a method for detecting cyanide content in milk and milk powder. Background technique [0002] Cyanide is a class of common highly toxic substances. Common inorganic cyanides include sodium cyanide, potassium cyanide and hydrogen cyanide. Cyanide decomposes into cyanide ions (CN - ), inhibit the activity of various enzymes in tissue cells, so that cells cannot use oxygen, resulting in intracellular suffocation, leading to central respiratory failure and death. my country's national standard "GB5749-2006 Hygienic Standard for Drinking Water" stipulates that the limit of cyanide is 0.05mg / L. Foreign countries have more detailed and strict limit requirements for cyanide in food. For example, Russia stipulates that the limit of cyanide in drinking water is 0.035mg / L. The food matrix puts forward the limit requirements: canned stone fruit is 5mg / kg, and othe...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 崔宗岩曹彦忠钱云开王飞贾光群张少博石秋玉黄学者
Owner INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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