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Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product

A technology of skin cells and cytokines, applied in skin care preparations, cosmetics, cosmetic preparations, etc., can solve the problems of not being able to guarantee whether there is still activity, not being able to improve the skin in an all-round way, and discounting product effects, so as to improve skin, Improve skin quality and ensure biological activity

Inactive Publication Date: 2016-06-29
深圳市真兴病毒技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the prior art, most of them are single or combination products of two cytokines, which directly lead to a relatively single product effect and cannot improve human skin in an all-round way.
At the same time, since the biologically active cell growth factor is directly added to the cosmetic base containing more complex ingredients, it is impossible to guarantee whether it will still be active after long-term mixing with the cosmetic base, and the effect of the product will be greatly reduced

Method used

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  • Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product
  • Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product
  • Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A factor composition for promoting skin cell growth, comprising EGF, bFGF and KGF, the mass ratio of EGF, bFGF and KGF is 2:1:1. The EGF stock solution, the bFGF stock solution and the KGF stock solution are mixed according to the above content ratios to obtain the skin cell growth-promoting factor composition.

[0031] The obtained above-mentioned factor composition promoting skin cell growth was added to NIH3T3 cells in an amount of 1 ng / ml, and the cell proliferation was observed after 48 hours. The results were as follows: figure 1 shown.

Embodiment 2

[0038] A compound freeze-dried powder for promoting the growth of skin cells, the quality of its main components is: EGF0.04g, bFGF0.02g, KGF0.02g, asiaticoside 25g, mannitol 250g, trehalose 500g.

[0039] Prepared using the following steps:

[0040] Step S1: mixing the EGF stock solution, the bFGF stock solution and the KGF stock solution, and adding asiaticoside to it to obtain a cytokine complex solution;

[0041] Step S2: preparing a lyoprotectant, adding mannitol and trehalose to deionized water, mixing them, and filtering through a 600-mesh filter membrane to obtain a coarse filtration solution of the lyoprotectant;

[0042] Step S3: Mix the cytokine complex solution and the lyoprotectant coarse filtration solution, add water to constant volume, filter and sterilize through a 0.22 μm filter membrane, and constant volume to 5000ml, aseptically pack into 1000 bottles of vials, 5ml / The bottle is half closed with a rubber stopper, and freeze-dried at a low temperature of -...

Embodiment 3

[0046] A compound freeze-dried powder for promoting the growth of skin cells, the quality of its main components is: EGF0.02g, bFGF0.009g, KGF0.011g, asiaticoside 50g, mannitol 500g, trehalose 250g.

[0047] Prepared using the following steps:

[0048] Step S1: mixing the EGF stock solution, the bFGF stock solution and the KGF stock solution, and adding asiaticoside to it to obtain a cytokine complex solution;

[0049] Step S2: preparing a lyoprotectant, adding mannitol and trehalose to deionized water, mixing them, and filtering through a 600-mesh filter membrane to obtain a coarse filtration solution of the lyoprotectant;

[0050] Step S3: Mix the cytokine complex solution and the lyoprotectant coarse filtration solution, add water to constant volume, filter and sterilize through a 0.22 μm filter membrane, and constant volume to 5000ml, aseptically pack into 1000 bottles of vials, 5ml / The bottle is half closed with a rubber stopper, and freeze-dried at a low temperature of...

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Abstract

The invention provides a factor composition and composite freeze-dried powder for promoting skin cell growth and a skin care product. The factor composition for promoting skin cell growth comprises EGF, bFGF and KGF; the composite freeze-dried powder for promoting skin cell growth is prepared from, by weight, 0.0003-0.0018% of the factor composition for promoting skin cell growth, 0.1-5% of asiaticoside, 5-28% of freeze-drying protecting agent and the balance water. By the adoption of the technical scheme, the three cell growth factors serve as ingredients for achieving main effects, and can achieve a synergistic effect to well promote growth of human body skin cells and improve the skin of people; through the freeze-drying process, bioactivity of the cell growth factors can be effectively guaranteed; the obtained skin care product has multiple effects and can comprehensively improve the skin of people.

Description

technical field [0001] The invention belongs to the technical field of biological genetic engineering, and in particular relates to a factor composition for promoting the growth of skin cells, a composite freeze-dried powder and a skin care product. Background technique [0002] In recent years, biogenetic engineering technology has made considerable progress, directly affecting all aspects of life sciences, and at the same time bringing new development opportunities to the beauty and cosmetics industry. Cosmetics have changed from traditional synthetic cosmetics and natural cosmetics to biogenetic cosmetics. Cosmetic development. In the era of biogenetic beauty cosmetics, a series of cell growth factors are mainly used as the main functional ingredients, which are directly added to skin care cream, body lotion, eye cream, skin care water, facial mask, repair essence and other cosmetics to achieve skin repair, anti-wrinkle, Light red bloodshot and other cosmetic effects. ...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/60A61K8/34A61K8/73A61Q19/00
CPCA61K8/64A61K8/345A61K8/602A61K8/735A61Q19/00
Inventor 何波吴泽进
Owner 深圳市真兴病毒技术有限公司
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