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Kit and method for detecting content of immune globulin E and application of kit

A technology for immunoglobulin and human immunoglobulin, applied in the field of detection of immunoglobulin E content, can solve the problems of low degree of automation, cumbersome determination process, and easy contamination, and achieve improved detection efficiency, simple and fast use, and high sensitivity high effect

Inactive Publication Date: 2016-06-29
潍坊三维生物工程集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The measurement process of radioimmunoassay (IRST) and enzyme-linked immunoassay is cumbersome, easily contaminated, time-consuming, poor in sensitivity, and not high in automation. It requires a variety of special equipment, which increases the cost to a certain extent

Method used

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  • Kit and method for detecting content of immune globulin E and application of kit
  • Kit and method for detecting content of immune globulin E and application of kit
  • Kit and method for detecting content of immune globulin E and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The kit for detecting immunoglobulin E comprises: reagent R1, reagent R2 and immunoglobulin E calibrator.

[0062] Wherein, reagent R1 comprises the following components:

[0063]

[0064]

[0065] Reagent R2 includes the following components:

[0066]

[0067] Immunoglobulin E calibrator includes the following components:

[0068]

[0069] Add the corresponding amount of immunoglobulin E antigen to the above-mentioned other reagent mixture according to the required concentration of the calibrator, and mix to obtain the immunoglobulin E calibrator. In this example, 6 immunoglobulin E calibrators with different concentrations were prepared, and the concentrations of the immunoglobulin E antigens were: 25IU / mL, 100IU / mL, 300IU / mL, 500IU / mL, 700IU / mL and 1000IU / mL . The immunoglobulin E calibrator was sterilized by filtration through a 0.22 μm filter membrane, and stored at 2-8°C.

[0070] Using the above kit, the samples to be tested were detected and anal...

Embodiment 2

[0072] The kit for detecting immunoglobulin E comprises: reagent R1, reagent R2 and immunoglobulin E calibrator.

[0073] Wherein, reagent R1 comprises the following components:

[0074]

[0075]

[0076] Reagent R2 includes the following components:

[0077]

[0078] Immunoglobulin E calibrator includes the following components:

[0079]

[0080] In this embodiment, a high-concentration single-point calibrator is selected, so the concentration of immunoglobulin E antigen is 1000 IU / mL. Then, the immunoglobulin E calibrator was sterilized by filtration through a 0.22 μm filter membrane, and stored at 2-8°C. When in use, dilute with normal saline into 6 different concentrations of immunoglobulin E calibrators, and the concentrations of immunoglobulin E antigens are: 25IU / mL, 100IU / mL, 300IU / mL, 500IU / mL, 700IU / mL and 1000IU / mL.

[0081] Using the above kit, detection and analysis were carried out by Hitachi 7080 automatic biochemical analyzer. The detection met...

Embodiment 3

[0083] The kit for detecting immunoglobulin E comprises: reagent R1, reagent R2 and immunoglobulin E calibrator.

[0084] Wherein, reagent R1 comprises the following components:

[0085]

[0086] Reagent R2 includes the following components:

[0087]

[0088]

[0089] Immunoglobulin E calibrator includes the following components:

[0090]

[0091] Add the corresponding amount of immunoglobulin E antigen to the above-mentioned other reagent mixture according to the required concentration of the calibrator, and mix to obtain the immunoglobulin E calibrator. In this example, 6 immunoglobulin E calibrators with different concentrations were prepared, and the concentrations of the immunoglobulin E antigens were: 25IU / mL, 100IU / mL, 300IU / mL, 500IU / mL, 700IU / mL and 1000IU / mL . The immunoglobulin E was sterilized by filtration through a 0.22 μm filter membrane, and stored at 2-8°C.

[0092] Using the above kit, detection and analysis were carried out by Hitachi 7080 au...

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Abstract

The invention discloses a kit for detecting the content of immune globulin E.The kit comprises a reagent R1, a reagent R2 and an immune globulin E calibrator; the reagent R1 is prepared from a first buffer solution, a first electrolyte, surfactant, a first stabilizer, a macromolecular accelerant and first preservative; the reagent R2 is prepared from a second buffer solution, latex particles coated with an anti-human immunoglobulin E antibody, a second electrolyte, a second stabilizer and second preservative; the immune globulin E calibrator is prepared from a third buffer solution, a third stabilizer, third preservative, antioxidant and an anti-human immunoglobulin E antigen.The invention further discloses application of the kit for detecting the content of immune globulin E to detection of the content of immune globulin E and a method of adopting the kit for detecting the content of immune globulin E to detect the content of immune globulin E.The kit and method for detecting the content of immune globulin E are used for detecting the content of immune globulin E and can simply and fast detect the content of immune globulin E.

Description

technical field [0001] The invention belongs to the technical field of detecting the content of immunoglobulin E, and in particular relates to a kit, method and application for detecting the content of immunoglobulin E. Background technique [0002] Immunoglobulin E (Immunoglobulin E, IgE) is mainly synthesized by B cells in the lamina propria lymphoid tissue of the respiratory tract and digestive tract mucosa, and is a mediator of allergic reactions. The content in the serum is very small, accounting for only 0.002% of the total serum immunoglobulin, but it is the main antibody that causes allergic reactions. [0003] IgE is a cytophilic antibody, and the serum content of normal people is extremely low, ranging from 10 to 350 U / ml. Serum IgE levels in patients with allergies and parasites were significantly higher than those in normal people. Since the discovery of IgE by Japanese scholar Ishizaka in 1966, a new era of allergic disease research has opened. The in vitro q...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6854
Inventor 夏德习曹慈崔晓辰王庆国宿明明
Owner 潍坊三维生物工程集团有限公司
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