Polypeptide iron chelate prepared from soy isolate protein serving as substrate and preparation method

A soybean protein isolate and chelate technology, applied in animal feed, animal feed, additional food elements, etc., can solve the problems of amino acid imbalance, low polypeptide content, high price, etc., and achieve high social and economic benefits, purity and content. The effect of improving and simplifying the production process

Inactive Publication Date: 2016-07-13
SHANGHAI YUANYAO BIOLOGICAL SHARES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are methods for preparing peptides or small peptides to chelate iron by enzymatically hydrolyzing some protein waste or soybean meal and cottonseed meal, but there are many problems in these methods, which will eventually lead to product quality problems
For example, a small peptide chelated trace element feed additive and its production process are patented (publication number: 102657285A). The amino acid in the hydrolyzed protein waste liquid used in it is unbalanced, and the heavy metals will exceed the standard. The pH8.0 is used for chelation, and the product contains A considerable part will exist in the form of iron hydroxide, and the spray drying temperature is too high, which will affect the product quality; patent a preparation method and application of a small peptide chelated iron complex (publication number: 102827908A), which uses fermented enzymatic hydrolysis of soybean meal, etc. After the protein raw material is chelated with metal salt

Method used

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  • Polypeptide iron chelate prepared from soy isolate protein serving as substrate and preparation method

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Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1: preparation is the polypeptide iron chelate of substrate with soybean protein isolate, comprises the following steps:

[0044] 1) Preparing medium: use soybean protein concentrate as the base material, prepare medium with ultra-fine powder soybean meal and glucose, the weight ratio of soybean protein concentrate: soybean meal: glucose is 85:5:10, soybean meal and glucose provide carbon source and Trace elements required for microbial growth, prepared and mixed evenly, and sterilized by steaming at 105°C;

[0045] 2) Fermentation stage: Prepare three-stage expansion culture strains, in which lactic acid bacteria: yeast = 1:3, and the inoculation concentration is 4×10 7 -9×10 7 cfu / ml, according to the ratio of material to water 1:2.0, mix with appropriate water and mix with the prepared medium evenly, control the fermentation temperature at 37-42°C, and the fermentation time is 42h;

[0046] 3) Enzymolysis stage: mix neutral protease and flavor protease w...

Embodiment 2

[0050] Embodiment 2: preparation is the polypeptide iron chelate of substrate with soybean protein isolate, comprises the following steps:

[0051] 1) Preparation of medium: use soybean protein concentrate as the base material, and prepare medium with ultrafine powder soybean meal and glucose, wherein the weight ratio of soybean protein concentrate: soybean meal: glucose is 85:10:5, and soybean meal and glucose provide carbon Sources and trace elements required for microbial growth, prepared and mixed evenly, and sterilized by steaming, the sterilization temperature is 105°C;

[0052] 2) Fermentation stage: Prepare three-stage expansion culture strains, in which lactic acid bacteria: yeast = 1:3, and the inoculation concentration is 4×10 7 -9×10 7 cfu / ml, according to the ratio of material to water 1:1.2-1:2.5, mix with appropriate water and mix with the prepared medium evenly, control the fermentation temperature at 37-42°C, and the fermentation time is 42h;

[0053] 3) Enz...

Embodiment 3

[0057] Embodiment 3: prepare the polypeptide iron chelate with soybean protein isolate as substrate, comprise the following steps:

[0058] 1) Preparation of medium: use soybean protein concentrate as the base material, and prepare medium with ultrafine powder soybean meal and glucose, wherein the weight ratio of soybean protein concentrate: soybean meal: glucose is 85:8:7, soybean meal and glucose provide carbon Sources and trace elements required for microbial growth, prepared and mixed evenly, and sterilized by steaming, the sterilization temperature is 105°C;

[0059] 2) Fermentation stage: Prepare three-stage expansion culture strains, in which lactic acid bacteria: yeast = 1:3, and the inoculation concentration is 4×10 7 -9×10 7 cfu / ml, according to the ratio of material to water 1:1.2-1:2.5, mix with appropriate water and mix with the prepared medium evenly, control the fermentation temperature at 37-42°C, and the fermentation time is 42h;

[0060] 3) Enzymolysis stag...

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Abstract

The invention discloses a polypeptide iron chelate prepared from soy isolate protein serving as a substrate and a preparation method of the polypeptide iron chelate. The polypeptide iron chelate is prepared from the soy isolate protein serving as a base material which is subjected to fermentation, enzymolysis, nanofiltration, chelation and drying, and polypeptide produced after fermentation, enzymolysis and filtration of the soy isolate protein is taken as a chelating agent for preparation of the polypeptide iron chelate. Compared with like polypeptide iron chelated iron, the purity of the polypeptide iron chelate is greatly improved and the content of the polypeptide iron chelate is greatly increased, the polypeptide iron chelate can completely replace pure microelement additives to be directly added to feed, and the application effect is good; the feed attractant effect is good; the product is stable and contains a large number of multifunctional polypeptide substances.

Description

technical field [0001] The invention relates to the field of feed production, in particular to a polypeptide iron chelate prepared with soybean protein isolate as a substrate and a preparation method thereof. Background technique [0002] Iron is one of the trace elements necessary for animals, and the manufacture of hemoglobin, myoglobin and cytochrome must rely on iron. Severe iron deficiency not only causes anemia, but also causes the lack of iron-containing enzymes in the body, which leads to the impairment of cellular respiration and affects the functions of tissues and organs. Clinically, dysfunction of the gastrointestinal tract, circulation, and nervous systems may occur (Lin Ping ,2004). The iron supplements traditionally used in pig feed are mainly inorganic iron (such as ferrous sulfate and ferrous chloride). Phytic acid, oxalic acid, phosphate ions) combine to form insoluble salts or precipitate out of the body, thereby reducing the absorption of metal ions. ...

Claims

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Application Information

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IPC IPC(8): A23K20/147A23K20/20A23K10/12A23K10/18
Inventor 郭子好方华季春源朱校适孙中超
Owner SHANGHAI YUANYAO BIOLOGICAL SHARES
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