Polypeptide zinc chelate prepared with soy protein isolate as base and preparation method

A soybean protein isolate and chelate technology, applied in animal feed, additional food elements, applications, etc., can solve the problems of amino acid imbalance, low polypeptide content, low polypeptide content, etc., and achieve high social and economic benefits, purity and content. Enhance and improve the effect of immune function

Inactive Publication Date: 2016-07-06
SHANGHAI YUANYAO BIOLOGICAL SHARES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are methods of enzymatically hydrolyzing some protein waste or soybean meal and cottonseed meal to prepare peptides or small peptides to chelate zinc, but there are many problems in these methods, which will eventually lead to product quality problems
For example, a small peptide chelated trace element feed additive and its production process are patented (publication number: 102657285A). The amino acid in the hydrolyzed protein waste liquid used in it is unbalanced, and the heavy metals will exceed the standard. The pH8.0 is used for chelation, and the product contains A considerable part will exist in the form of zinc hydroxide, and the spray drying temperature is too high, which will affect the product quality; patent a preparation method and application of a small peptide chelated zinc complex (publication number: 102827909A), which uses fermented enzymatic hydrolysis of soybean meal, etc. After the protein raw material is chelated with metal salts, t

Method used

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  • Polypeptide zinc chelate prepared with soy protein isolate as base and preparation method

Examples

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Example Embodiment

[0041] Example 1: Preparation of a polypeptide zinc chelate with soybean protein isolate as a substrate, including the following steps:

[0042] 1) Preparation of culture medium: The medium is prepared with soybean protein concentrate as the base material, and superfine powdered soybean meal and glucose. The weight ratio of soybean protein concentrate: soybean meal: glucose is 85:5:10, and soybean meal and glucose provide carbon source and The trace elements required for the growth of microorganisms are prepared and mixed uniformly, and sterilized by steaming at a temperature of 105°C

[0043] 2) Fermentation stage: prepare three-stage expansion culture strains, in which lactic acid bacteria: yeasts = 1:3, and the inoculation concentration is 4×10 7 -9×10 7 cfu / ml, mix with appropriate water according to the material-to-water ratio of 1:2.0 and mix it with the equipped medium evenly, control the fermentation temperature at 37-42℃, and the fermentation time for 42h;

[0044] 3) Enzyma...

Example Embodiment

[0048] Example 2: Preparation of a polypeptide zinc chelate with soybean protein isolate as a substrate, including the following steps:

[0049] 1) Preparation of culture medium: The medium is prepared with soybean protein concentrate as the base material, and superfine powdered soybean meal and glucose. The weight ratio of soybean protein concentrate: soybean meal: glucose is 85:10:5, and soybean meal and glucose provide carbon. Sources and trace elements required for the growth of microorganisms are prepared and mixed uniformly, and sterilized by steaming at 105°C;

[0050] 2) Fermentation stage: prepare three-stage expansion culture strains, in which lactic acid bacteria: yeasts = 1:3, and the inoculation concentration is 4×10 7 -9×10 7 cfu / ml, mix with appropriate water according to the material-to-water ratio 1:1.2-1:2.5 and mix it with the equipped medium evenly, control the fermentation temperature at 37-42℃, and the fermentation time for 42h;

[0051] 3) Enzymolysis stage: Us...

Example Embodiment

[0055] Example 3: Preparation of a polypeptide zinc chelate with soybean protein isolate as a substrate, including the following steps:

[0056] 1) Preparation of culture medium: The medium is prepared with soybean protein concentrate as the base material, and superfine powdered soybean meal and glucose. The weight ratio of soybean protein concentrate: soybean meal: glucose is 85:8:7, and soybean meal and glucose provide carbon. Sources and trace elements required for the growth of microorganisms are prepared and mixed uniformly, and sterilized by steaming at 105°C;

[0057] 2) Fermentation stage: prepare three-stage expansion culture strains, in which lactic acid bacteria: yeasts = 1:3, and the inoculation concentration is 4×10 7 -9×10 7 cfu / ml, mix with appropriate water according to the material-to-water ratio 1:1.2-1:2.5 and mix it with the equipped medium evenly, control the fermentation temperature at 37-42℃, and the fermentation time for 42h;

[0058] 3) Enzymolysis stage: Use...

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Abstract

The invention discloses polypeptide zinc chelate prepared with soy protein isolate as base and a preparation method. The polypeptide zinc chelate is prepared by soy protein isolate as base through fermenting, enzymolysis, nanofiltering, chelating and drying. After fermenting, enzymolysis and filtering of the soy protein isolate, polypeptide is generated as chelate to obtain polypeptide zinc chelate.Purity and content of the polypeptide zinc chelate are higher than those of the like polypeptide chelate zinc, and the polypeptide zinc chelate can be directly added into fodder completely instead of pure microelement additive and is excellent in application effect. The polypeptide zinc chelate is good in food calling effect, stable and contains much multifunctional polypeptide.

Description

technical field [0001] The invention relates to the field of feed production, in particular to a polypeptide zinc chelate prepared with soybean protein isolate as a substrate and a preparation method thereof. Background technique [0002] Zinc is an essential trace element in animals. It is related to the activity of nearly 300 enzymes in the body. Some enzymes use zinc as their structural component, and some use zinc as their cofactor. Zinc regulates and controls the structure and function of these enzymes. function, thereby affecting many metabolic functions of the body; at the same time, zinc can also change the structure and function of the epithelial cells of the digestive tract, regulate the activity of various zinc-containing digestive enzymes, and affect the digestion of pigs (Calm, 2003). The zinc supplements traditionally used in pig feed are mainly inorganic zinc (such as zinc sulfate, zinc oxide). Oxalic acid, phosphate ions) combine to form insoluble salts or p...

Claims

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Application Information

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IPC IPC(8): A23K20/20A23K20/142
Inventor 郭子好方华季春源朱校适孙中超
Owner SHANGHAI YUANYAO BIOLOGICAL SHARES
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