Polypeptide fishy-smell-removing method

A deodorizing and alcohol-soluble technology, applied in the field of deep processing of scallops, can solve the problems of heavy fishy smell, restricted application, poor product flavor, etc., and achieves the effects of increasing purity and content, enhancing hydrophobicity, and improving deodorizing effect.

Inactive Publication Date: 2018-10-12
金华市铁骑士生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, like other protein hydrolysates, the scallop by-product hydrolyzate has a strong fishy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1:

[0018] A method for deodorizing polypeptides includes the following steps:

[0019] 1) Dialysis the scallop enzymatic hydrolysate with a 10ku pore diameter fiber dialysis bag. The solid-liquid ratio in the enzymatic hydrolysate is 1:150, the temperature is 1°C, and the dialysate is changed for 72 hours. The dialysate is changed every 24 hours and stored at low temperature; 2) The enzymatic hydrolysate after dialysis passes through the ultrafiltration membrane into polysulfone hollow fiber, the effective membrane area is 0.3m 2 , The inlet pressure is 0.13MPa, the outlet pressure is 0.05MPa, the working temperature is 35℃, and the pH is 7, collect the permeate, return the permeate to the circulating storage tank and perform ultrafiltration again until there is no impermeable component to obtain the peptide solution 3) Add 0.1‰ of ethyl maltol and 0.01‰ of β-cyclodextrin to the peptide solution after the ultrafiltration is completed, and then let it stand for 36h ...

Example Embodiment

[0020] Example 2:

[0021] A method for deodorizing polypeptides includes: enzymatic hydrolysis, dialysis, ultrafiltration deodorizing, masking deodorizing, and alcohol soluble deodorizing, which specifically includes the following steps:

[0022] 1) Enzymatic hydrolysis: After cooking the fresh scallops, remove the shells from the shells, put the remaining by-products in a pot, cook for 45 minutes, and pour into a homogenizer to fully homogenize as substrates for enzymatic hydrolysis; In a constant temperature water bath, add 3000U / g neutral protease and 3500U / g papain, enzymatically hydrolyze under the conditions of 1g:8mL, temperature of 50℃ and pH of 7 for 5 hours, and then adjust the pH to 6. Add 1500U / g flavor protease to continue enzymatic hydrolysis for 3 hours, and inactivate the enzyme in a boiling water bath for 20 minutes. After the enzymatic hydrolysate is cooled to room temperature, centrifuge at 4000r / min for 30 minutes, and the supernatant obtained is the scallop en...

Example Embodiment

[0027] Example 3:

[0028] A method for deodorizing polypeptides, including: dialysis, ultrafiltration, deodorizing, masking, and alcohol soluble deodorizing, specifically including the following steps:

[0029] Dialysis: Dialysis of the scallop hydrolysate with a 10ku aperture fiber dialysis bag. The solid-liquid ratio in the enzymatic hydrolysate is 1:160, the temperature is 1.5℃, and the dialysate is changed for 84 hours. The dialysate is changed every 24 hours and stored at low temperature. Effectively reduce the ash content in scallop peptides, the ash content can be reduced to less than 3%, and the purity and content of low-molecular-weight proteins and peptides in the enzymatic hydrolysate are increased;

[0030] Ultrafiltration and deodorization: pass the dialysis enzymatic hydrolysate through an ultrafiltration membrane into polysulfone hollow fiber, the effective membrane area is 0.4m 2 , The inlet pressure is 0.14MPa, the outlet pressure is 0.06MPa, the working temperature...

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PUM

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Abstract

The invention discloses a polypeptide fishy-smell-removing method. The polypeptide fishy-smell-removing method comprises the steps of dialysis, ultra-filtration fishy smell removing, shielding fishy smell removing and alcohol-soluble fishy smell removing. Firstly, a chlamys farreri enzymatic hydrolysate is dialysed to remove ash content; then the enzymatic hydrolysate is subjected to ultra-filtration fishy smell removing through a polysulfone hollow fiber ultra-filtration membrane; then ethyl maltol, beta-cyclodextrin and pantolactone are added into a peptide solution subjected to ultra-filtration for shielding fishy smell removing; and finally, an ethanol solution is added for centrifuging to take sediment, and then polypeptides from chlamys farreri are obtained. The polypeptide fishy-smell-removing method has the beneficial effects that the polypeptide fishy-smell-removing method is easy to operate, fishy smell substances of amine, aldehyde, ketone and the like in a polypeptide solution are removed thoroughly, adsorption and wrapping of the beta-cyclodextrin for the aldehyde, ketone and amine type substances can be greatly improved through the pantolactone, 3-hydroxy-5-methyl-2,4-imidazolone in alcohol-soluble fishy smell removing facilitates acceleration separating of the aldehyde and amine type substances, and meanwhile degradation of the polypeptides and proteins can be lowered.

Description

technical field [0001] The invention relates to the field of deep processing of scallops, in particular to a method for deodorizing polypeptides. technical background [0002] Scallop is the synonym for the bivalve mollusk of the genus Scallop, and there are about 400 species. More than 60 species of this family are one of the important marine fishery resources all over the world. The shell, meat and nacre have extremely high utilization value. Many scallops are eaten as a delicacy. Scallops are generally not exposed when the sea water is low tide, so it is more troublesome to catch them. On the coast of my country, scallop fishing is mainly in the north, and only Dongchu Island, a little north of Shidao in Shandong Province, and Changshan Islands in Changhai County, Liaoning Province are the most famous. Scallop shells have various colors and neat and beautiful ribs, so they are good materials for making shell carving crafts. People who go to the beach to work, travel ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34A23L33/18A23L5/20
CPCC12P21/06A23L5/20A23L5/27A23L5/273A23L33/18A23V2002/00A23V2250/55
Inventor 全盈园
Owner 金华市铁骑士生物科技有限公司
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