Processing method for extracting composite active polypeptide by selecting fresh cornu cervi pantotrichum

Abstract

The invention discloses a processing method for extracting composite active polypeptide by selecting fresh cornu cervi pantotrichum. The processing method comprises the steps of: carrying out preliminary cleaning, blood water removal, skin and hair removal, slicing and other pretreatment on the cornu cervi pantotrichum; carrying out vibration lavaging on the slices to separate cell suspension and matrix residues; then respectively carrying out extraction on the cell suspension and the matrix residues; and then forming a small molecule peptide structure easier to absorb through enzymolysis, wherein in a mode of carrying out refined extraction on the cell suspension and carrying out repeated crude extraction on the matrix residues, the content and the purity of the cornu cervi pantotrichum are improved, and meanwhile, various synergistic gain components are retained.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a processing method for extracting complex active polypeptides from fresh deer antler. Background technique [0002] Joint disease is a common disease in modern people, especially with the change of work status, office workers are in a long-term sedentary, high-work pressure, long-term work state, and more and more people often have joint abnormalities status. Sitting at a desk for a long time will easily lead to less lubricating fluid produced between joints and increase the loss of joint friction. Over time, it will increase the wear and tear on cartilage, and excessive cartilage wear may cause degenerative arthritis and increase the burden on the body. [0003] Articular cartilage is a special connective tissue that covers the load-bearing articular surface of joints. Articular cartilage is composed of matrix and chondrocytes to maintain functions. The matrix mainly contains wat...

AI Technical Summary

Problems solved by technology

[0006] (1) Traditionally, the velvet polypeptide obtained by heating, strong acid or enzymatic proteolysis of fresh velvet, although this method can obtain velvet polypeptide with higher content and higher yield, but its preparation conditions make the obtained velvet polypeptide in a large To a certain extent, the natural biological activity is lost, which greatly reduces the biological application effect; (2) conventionally, the velvet antler polypeptide obtained by extracting and separating by the acid alcohol method has more retention of molecular conformation and natural characteristics than traditional methods, and its biological activity is also somewhat However, the velvet antler polypeptide obtained by the acid alcohol method is relatively rough, the purity is not high, and contains more insoluble impurities, corticosteroid hormones and sex hormones, and these direct applications have allergic risks and other side effects; (3) column chromatography or high-efficiency Further purification in the liquid phase, although antler polypeptides with better molecular weight distribution uniformity, higher purity, and better biological activity can be obtained, but other ingredients, including functional ingredients with synergistic effects, are eliminated, making it more effective in practical applications. Great discount; in addition, velvet polypeptide is not easy to preserve in liquid, and is easy to degrade and oxidize, and its biological activity is also greatly reduced