Processing method for extracting composite active polypeptide by selecting fresh cornu cervi pantotrichum

An active polypeptide and processing method technology, applied in the biological field, can solve the problems of low purity, reduced biological activity, discounted effect, etc., and achieve the effects of improving content and purity, easy absorption, and promoting growth.

Pending Publication Date: 2021-08-20
深圳中正生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) Traditionally, the velvet polypeptide obtained by heating, strong acid or enzymatic proteolysis of fresh velvet, although this method can obtain velvet polypeptide with higher content and higher yield, but its preparation conditions make the obtained velvet polypeptide in a large To a certain extent, the natural biological activity is lost, which greatly reduces the biological application effect; (2) conventionally, the velvet antler polypeptide obtained by extracting and separating by the acid alcohol method has more retention of molecular conformation and natural characteristics than traditional methods, and its biological activity is also somewhat However, the velvet antler polypeptide obtained by the acid alcohol method is r

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example one

[0024]Choose 100g fresh antler as a raw material, 1 Wash the fresh antler cleans the outer surface through pure water, scrape the surface of the fluff, washing the fresh deer fraction of 75% ethanol, cut into a thickness of 1.4 CM block, with 25 ° C, the volume fraction is 85% ethanol 48h, washed with water, drained, cut into 0.35 sheets; 2 Put the velvet slice according to the sheet interval in the cleaning tube, will clean the tube Open, the exhaust hole of the other end of the tube is connected to the pump, after the cleaning tube is vacuished, the cleaning solution connected to the irrigation port is filled into the cleaning tube filled with the velvet flakes, and the cleaning liquid uses 200ml mass concentration of 0.2. % SDS solution or a mass concentration of 0.2% Tritonx-100 solution, closing the glow tube back to the cleaning tube back to the cleaning tube, the vibration frequency is 20 kHz, wherein the spacing between the sheet and the sheet is 3 cm,...

Example Embodiment

[0025] Example 2

[0026] Choose 100g fresh antler as a raw material, 1 Wash the fresh antler cleans the outer surface through pure water, scrape the surface of the fluff, cleaning the fresh deer fragrance with a volume fraction of 75% ethanol, cutting the thickness of 1.5 CM block, the volume fraction of 20 ° C is 85% of ethanol in ethanol 48h, washed with water, drained, cut into 0.75 cm sheet; 2 Put the velvet flakes in the cleaning tube according to the tablet interval, will clean the tube The spilling mouth opens, the exhaust hole of the other end of the tube is connected to the pump, after the cleaning tube is vacuished, the cleaning liquid connected to the glow is filled into the cleaning tube filled with the velvet thin film, and the cleaning liquid uses 200 ml of mass concentration. 0.2% of the SDS solution, the cleaning tube was turned back to the cleaning tube back to the cleaning tube and the vibration frequency was 20 Hz, wherein the spacing void between the sheets an...

Example Embodiment

[0027] Example three

[0028] Choose 100g fresh antler as a raw material, 1 Wash the fresh antler cleans the outer surface through pure water, scrape the surface of the fluff, cleaning the fresh deer fraction of 75% ethanol and disinfect, cutting the thickness of 1cm Block, with 25 ° C, the volume fraction is 85% ethanol 48h, washed with water, drained, cut into 0.5 sheets; 2 Put the velvet flakes in the cleaning tube according to the sheet interval, and the water purity The liquid port is opened, the exhaust hole of the other end of the tube is connected to the pump, after the cleaning tube is vacuished, the cleaning solution connected to the glow is inserted into the cleaning tube filled with velvet, the cleaning liquid uses 200ml mass concentration of 0.2%. The Tritonx-100 solution, the cleaning tube is turned back to the cleaning tube back and vibrates 10-15 min, and the vapor frequency is 20 kHz, wherein the spacing between the sheets and the sheets is 3 cm, so that the sheet...

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PUM

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Abstract

The invention discloses a processing method for extracting composite active polypeptide by selecting fresh cornu cervi pantotrichum. The processing method comprises the steps of: carrying out preliminary cleaning, blood water removal, skin and hair removal, slicing and other pretreatment on the cornu cervi pantotrichum; carrying out vibration lavaging on the slices to separate cell suspension and matrix residues; then respectively carrying out extraction on the cell suspension and the matrix residues; and then forming a small molecule peptide structure easier to absorb through enzymolysis, wherein in a mode of carrying out refined extraction on the cell suspension and carrying out repeated crude extraction on the matrix residues, the content and the purity of the cornu cervi pantotrichum are improved, and meanwhile, various synergistic gain components are retained.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a processing method for extracting complex active polypeptides from fresh deer antler. Background technique [0002] Joint disease is a common disease in modern people, especially with the change of work status, office workers are in a long-term sedentary, high-work pressure, long-term work state, and more and more people often have joint abnormalities status. Sitting at a desk for a long time will easily lead to less lubricating fluid produced between joints and increase the loss of joint friction. Over time, it will increase the wear and tear on cartilage, and excessive cartilage wear may cause degenerative arthritis and increase the burden on the body. [0003] Articular cartilage is a special connective tissue that covers the load-bearing articular surface of joints. Articular cartilage is composed of matrix and chondrocytes to maintain functions. The matrix mainly contains wat...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/34C07K1/16C07K1/36C12P21/06
CPCC07K1/145C07K1/34C07K1/16C07K1/36C12P21/06Y02A50/30
Inventor 胡铁峰
Owner 深圳中正生物技术有限责任公司
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