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Extracting method for carotenoid in corn flour

A technology of carotene and extraction method, applied in the direction of organic chemistry, etc., can solve the problem of low carotenoid content, achieve the effect of high extraction rate, low equipment requirements, and simple operation

Inactive Publication Date: 2016-07-13
赵雪兰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a method for extracting carotenoids in corn flour in order to overcome the problem of low content of carotenoids extracted from corn flour in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) After the corn flour grains are harvested, dry them in the sun, grind them with a Brabender Quandrmat Junior experimental mill, and pass through a 60-mesh sieve, and store the ground and sieved powder samples in the dark at -20°C.

[0024] (2) Weigh 8.0g of powdered sample into a 100ml blue cap reagent bottle, add 40.0ml of acetone:ethyl acetate=8:2 extract, add 0.01% of 2,6-di-tert-butyl-p-cresol (BHT), cap tightly and mix on a mixer to fully wet the powdered sample.

[0025] (3) Place the mixed powdery sample in a constant temperature shaker at 300 rpm and 35° C. for 1 hour.

[0026] (4) After shaking, let stand for a while, transfer the supernatant to a 50ml centrifuge tube, and centrifuge at 23,500xg at 4°C for 15 minutes.

Embodiment 2

[0028] (1) After the corn flour grains are harvested, dry them in the sun, grind them with a Brabender Quandrmat Junior experimental mill, and pass through a 60-mesh sieve, and store the ground and sieved powder samples in the dark at -20°C.

[0029] (2) Weigh 9.0g of powdered sample into a 100ml blue cap reagent bottle, add 40.0ml of acetone: ethyl acetate = 8:2 extract, add 0.01% 1,2,3-glucinol, cover Tighten the stopper and mix on a mixer to fully wet the powdered sample.

[0030] (3) Place the mixed powdery sample in a constant temperature shaker at 300 rpm and 35° C. for 1 hour.

[0031] (4) After shaking, let stand for a while, transfer the supernatant to a 50ml centrifuge tube, and centrifuge at 23,500xg at 4°C for 15 minutes.

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PUM

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Abstract

The invention discloses an extracting method for carotenoid in corn flour. The method comprises the following specific steps: mixing mixed liquor of acetone and ethyl acetate with the corn flour, wherein extracting liquor of acetone-ethyl acetate contains 0.01%-1% (w / v) of an antioxidant; and oscillating the extracting liquor at a constant temperature to finally extract the carotenoid in corn flour. The method for extracting carotenoid from corn flour has the advantages of being convenient and quick, simple to operate, and low in equipment requirement, and is high in extracting rate of the carotenoid.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, in particular to a method for extracting carotenoids in corn flour. Background technique [0002] Corn is the second largest cereal crop in the world. Corn flour is rich in potassium, phosphorus, iron, zinc, calcium, VB1, VB5, VB6, and rich in carotene pigments. Since corn flour does not contain gluten protein, corn flour cannot be used alone to make steamed buns or bread. Corn flour is often added to flour to make steamed buns or bread. In recent years, many countries in Europe and the United States have approved the use of zeaxanthin as food coloring, and companies such as Roche Pharmaceuticals in Switzerland and DSM in the Netherlands have launched related health care and treatment products. But in today's fast-changing food industry, people's health awareness is constantly improving, and new standards for food safety have also been proposed. Although synthetic pigments have bright ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
CPCC07C403/24
Inventor 赵雪兰
Owner 赵雪兰