Bletilla striata mycorrhizal fungus and application thereof

A mycorrhizal fungus and mycelium technology, applied in the fields of application, fungi, chemicals for biological control, etc., can solve problems such as difficulty in meeting cultivation needs, susceptibility to pathogenic bacteria, and slow propagation of rhizome ramets, etc. Achieve the effects of protection, regeneration and sustainable utilization, shorten the growth cycle, and achieve remarkable cultivation effects

Active Publication Date: 2016-07-13
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional rhizome ramets have slow propagation speed, long growth cycle, low efficiency, high cost of seedlings, and it is difficult to meet the needs of large-scale cultivation; while sterile test-tube seedlings produced by tissue culture grow slowly, have low transplanting survival rate, and are resistant to stress. Poor sex, susceptible to pathogenic bacteria
Judging from the current situation, the difficulty in reproduction of white and white has not been fundamentally resolved, resulting in the near exhaustion of wild white and wild resources, and the market is in short supply, which seriously limits the demand for clinical medicine and industrial production of white and
[0006] In the prior art, there is no report on the application of fungi for promoting germination of Radix Radix Radix in the field. Therefore, screening and utilizing fungi that can promote the germination of Radix Radix Radix Radix Radix Radix Radix Radix L. is an effective way to realize the field cultivation of Orchidaceae. For It has important practical significance and application value to raise the seedlings and cultivation of Baiji, improve the quality and output of Baiji medicinal materials, and protect the resources of Baiji.

Method used

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  • Bletilla striata mycorrhizal fungus and application thereof
  • Bletilla striata mycorrhizal fungus and application thereof
  • Bletilla striata mycorrhizal fungus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Identification of strains

[0039] 1. Extraction of fungal DNA:

[0040] The fungus used for molecular identification was inoculated on PDA medium and cultured in a dark room at 25°C for 2 weeks. When the diameter of the fungal colony was 3-5 cm, DNA could be extracted from the hyphae on the surface of the colony.

[0041] DNA extraction was performed according to the instructions of the fungal DNA extraction kit (E.Z.N.ATMFungalDNAKit, OmegaBio-Tek, Doraville, Georgia, USA), and the specific steps were as follows:

[0042] 1) Scrape 50-100 mg mycelium into a sterilized mortar, add liquid nitrogen to freeze, grind it into powder immediately, and scrape the ground mycelium into a sterilized 1.5mL Eppendorf tube.

[0043] 2) Add 800 μL of BufferFG1 buffer solution into the Eppendorf tube, vortex to mix, and then put in a water bath at 65°C for 10 minutes, and invert and mix twice during the water bath.

[0044] 3) Then add 140 μL of BufferFG2, vortex and mix well, and t...

Embodiment 2

[0080] 1. First, use PDA solid medium to activate the mycorrhizal fungus strains. The culture temperature is 26±2°C, culture in dark for 7 to 15 days, and then pick a few small pieces from the edge of the formed colony and inoculate them into the liquid medium Carry out dark cultivation on a shaking table, the rotating speed of the shaking table is 100r / min-150r / min, the cultivation temperature is 26°C±2°C, and the dark cultivation is carried out for 10-20 days. After the cultivation, the obtained mycorrhizal fungus mycelium can be directly used. It can also be used after crushing with a soymilk machine.

[0081] 2. Effect of liquid culture medium on mycorrhizal fungus mycelium

[0082] The liquid medium is composed of glucose, inorganic salts and natural extracts, wherein the amount of glucose added is 15g / L-40g / L, and the inorganic salt is MgSO 4 ·7H 2 O and KH 2 PO 4 , the natural extract is one of potato extract, wheat bran extract and oat extract.

[0083] 1) For MgS...

Embodiment 3

[0107] The inventor took 200 Radix Radix and divided them into two groups on average. In Group 1, according to the method in Example 2, Radix Radix Radix and Radix Radix Radix Mycorrhizae were used as materials to obtain Radix Radix Radix Radix Radix Seed and Radix Radix Radix Rhizome. After the symbiotic mixture of the fungus, it was directly sown in the field, and in the 2 groups, it was directly sown in the field with the seeds of Baiji. In the 5th week after sowing, the seeds in the 1 group began to germinate, and the germination of the seeds in 5 to 12 weeks was measured, as shown in Table 5. Show:

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Abstract

The invention discloses a Bletilla striata mycorrhizal fungus which is Sebacinales sp.mycorrhizal fungal strain YY-51 and is collected in China General Microbiological Culture Collection Center on 31th July, 2015, at No.3, Institute 1, Beichen western road, Chaoyang District of Beijing, in Institute of Microbiology, Chinese Academy of Sciences, under GCMCC NO.11104.Mycelia of the Bletilla striata mycorrhizal fungus and Bletilla striata seeds can be made into a Bletilla striata seed and Bletilla striata mycorrhizal fungus symbiotic mixture to facilitate Bletilla striata seed germination, with germinating rate up to 98%.

Description

technical field [0001] The present invention relates to the field of biotechnology. More specifically, the present invention relates to a mycorrhizal fungus and its application. Background technique [0002] Orchidaceae is a relatively large family of angiosperms, including many rare and valuable medicinal plants and flowers, such as Dendrobium candidum, Clematis, Baiji, etc. It is also a special ecology with a high degree of evolution in the plant kingdom and is closely related to fungi. Taxa. Whether it is seed germination, seedling growth, or plant formation of orchid plants, mycorrhizal fungi are inseparable. Orchid mycorrhizae are particularly important in the protection, introduction, domestication and artificial cultivation of orchid medicinal and ornamental plants. However, since most orchids are endangered, all wild species of orchids have been listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora, which is a "flagship" grou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00A01C1/02C12R1/645
CPCA01C1/02A01N63/30C12N1/145C12R2001/645
Inventor 谭小明周雅琴缪剑华余丽莹
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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