Antrodia cultivating method capable of improving content of triterpene

A cultivation method and content technology, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problem of low cultivation cost, and achieve the effect of simple cultivation method, lower production cost, and easy acquisition

Inactive Publication Date: 2016-07-20
FUJIAN HUASHEN ANTRODIA CAMPHORATA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the artificial cultivation method of the above-mentioned traditional Antrodia camphorata, it is necessary t

Method used

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  • Antrodia cultivating method capable of improving content of triterpene
  • Antrodia cultivating method capable of improving content of triterpene
  • Antrodia cultivating method capable of improving content of triterpene

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Experimental program
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Example Embodiment

[0047] Example 1

[0048] An Antrodia cinnamomea cultivating method capable of increasing triterpene content, which comprises the following steps:

[0049] Step 10): Preparation of the culture medium, which includes the preparation of the mother seed culture medium, and the preparation of the original seed or cultivated seed culture medium, specifically:

[0050] The preparation of the mother seed culture medium includes the following steps:

[0051] 11) Weigh the following components by weight:

[0052]

[0053] 12) Boil the substrate with water for 20 minutes, filter, add agar, potassium dihydrogen phosphate, magnesium sulfate and vitamin B1 to the filter cake, heat and stir to dissolve all the solids, then add glucose to the solution, stir to dissolve and add water to Make up the solution to 1000 parts by weight;

[0054] 13) Then divide the solution into test tubes, each tube is filled with 1 / 4-1 / 3 of the height of the test tube, plugged with cotton plug, sterilized at 110°C for 35 ...

Example Embodiment

[0072] Example 2

[0073] An Antrodia cinnamomea cultivating method capable of increasing triterpene content, which comprises the following steps:

[0074] Step 10): Preparation of bacterial culture medium, including preparation of mother seed culture medium, and preparation of original seed or cultivated seed culture medium, specifically

[0075] The preparation of the mother seed culture medium includes the following steps:

[0076] 11) Weigh the following components by weight:

[0077]

[0078] 12) Boil the base material with water for 30 minutes, filter, add agar, potassium dihydrogen phosphate, magnesium sulfate and vitamin B1 to the filter cake, heat and stir to dissolve all the solids, then add glucose to the solution, stir to dissolve, add water Make up the solution to 1000 parts by weight;

[0079] 13) Then divide the solution into test tubes, each tube is 1 / 4-1 / 3 of the height of the test tube, plugged with cotton plug, sterilized at 130°C for 25 minutes, taken out and placed ...

Example Embodiment

[0097] Example 3

[0098] An Antrodia cinnamomea cultivating method capable of increasing triterpene content, which comprises the following steps:

[0099] Step 10): Preparation of bacterial culture medium, including preparation of mother seed culture medium, and preparation of original seed or cultivated seed culture medium, specifically

[0100] The preparation of the mother seed culture medium includes the following steps:

[0101] 11) Weigh the following components by weight:

[0102]

[0103]

[0104] 12) Boil the substrate with water for 25 minutes, filter, add agar, potassium dihydrogen phosphate, magnesium sulfate and vitamin B1 to the filter cake, heat and stir to dissolve all the solids, then add glucose to the solution, stir to dissolve, add water to Make up the solution to 1000 parts by weight;

[0105] 13) Then the solution is divided into test tubes, each tube is filled with 1 / 4-1 / 3 of the height of the test tube, plugged with cotton plug, sterilized at 120°C for 30 minute...

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Abstract

The invention discloses an antrodia cultivating method.The method comprises the steps of strain culture medium manufacturing, strain isolation and expanding propagation, carrier cultivating, inoculation, mycelial growth and sporocarp growth.The antrodia cultivating method has the advantages that due to the cultivating method is simple and capable of achieving industrialization and standardized production, so that expanded production is promoted, the production cost is lowered, and the cultivating time is short.Low-density wood is adopted as a bacteria bed, mycelial growth is promoted, hyphae can easily form sporocarp, and meanwhile strains can better adapt to bacteria bed growth by improving the strains, so that the fruiting rate can be improved by about 80%, and economic benefits can be improved.The obtained antrodia sporocarp and wild antrodia sporocarp DNA detection equal rate can reach 99%, and the effect is good.The cultivating method can enable the content of triterpene of the antrodia sporocarp can reach 8.6%, the content of polysaccharide can reach 4.8%, and the content of effective elements can be improved.

Description

technical field [0001] The invention relates to the field of fungi cultivation, in particular to a method for cultivating Antrodia camphorata that can increase the content of triterpenes. Background technique [0002] Antrodia cinnamomea, also known as camphor mushroom, is a precious medicinal fungus that grows on camphor trees. Antrodia cintrodia contains polysaccharides, terpenoids, superoxide dismutase and nucleic acids, etc. It has detoxification, liver protection, anti Cancer, immune strengthening, anti-allergic, blood fat-lowering and many other effects are greatly welcomed by people. Antrodia antrodia fungus has strong selectivity to the substrate, and is a typical and highly specific wood-rot fungus. [0003] At present, the artificial cultivation methods of Antrodia camphorata include camphor tree basswood cultivation method, solid-state cultivation method and liquid fermentation method. The solid-state cultivation method is to cultivate the mycelium of Antrodia c...

Claims

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Application Information

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IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 马建煜
Owner FUJIAN HUASHEN ANTRODIA CAMPHORATA BIOTECH CO LTD
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