Full-automatic double-layer tissue culture room

Abstract

The invention provides a full-automatic double-layer tissue culture room, and belongs to the field of plant tissue culture equipment.The full-automatic double-layer tissue culture room comprises an outer room body, an inner room body and a control system.The outer room body comprises a dressing room and a sterilizing system.The inner room body comprises a tissue culture rack, a detecting system, a temperature control system, a tissue culture light source system and an illuminating system.The control system comprises a PLC control system and an upper computer.Ultraviolet irradiation is adopted in the outer room body for a long time for sterilization.When getting in and out, technicists can be slowly flushed in the outer room body firstly, put on sterile garments and then enter the inner room body, the probability of bringing outer substances adverse to the tissue culture environment into the tissue culture room is reduced to a certain degree, the control system is used for uniformly controlling the tissue culture room, and real-time, accurate and automatic control can be achieved.The full-automatic double-layer tissue culture room has the advantages of being automatic, high in sterilization efficiency and low in environmental bacterium density.

Description

technical field [0001] The invention belongs to the field of plant tissue culture equipment, in particular to a fully automatic double-layer tissue culture room. Background technique [0002] Tissue culture technology is to place living organs, tissues or cells in the culture medium under sterile conditions and place them in a suitable environment for continuous culture of cells, tissues or individuals. It mainly uses the totipotency of cells, That is, each cell has a complete set of genetic material, and the differentiated plant tissue is induced to dedifferentiate through the special combination of various hormones in the medium, thereby inducing callus, and further inducing new individuals. This technology has been widely used in the research of agriculture, biology and medicine, and has made great contributions to the rapid propagation of plants, the protection of plant species and related research. [0003] However, since the medium used in tissue culture contains a la...

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Problems solved by technology

[0003] However, since the medium used in tissue culture contains a large amount of nutrients, it is not only suitable for the growth of plant cells and tissues, but also suitable for the growth of various bacteria and fungi. The growth of bacteria will consume a large amount of nutrients in the medium, which is harmful to plants. influence on cell growth

In addition, due to the low degree of callus differentiation and poor resistance to bacteria and fungi, it is extremely vulnerable to the substances released by bacteria and fungi, or is poisoned or mutated, which has a great impact on the tissue culture industry. Therefore, a clean and sterile environment is extremely important for tissue culture

[0004] The tissue culture room is an important means to control the tissue culture environment, but the current tissue culture room is mostly an ordinary room. When technicians enter and exit, it is inevitable that bacteria, fungi and other substances that are not conducive to the tissue culture environment will be brought into the tissue culture. Indoors, which affect the effect of tissue culture, and relying on manual monitoring and control of environmental changes in the tissue culture room often cannot make timely adjustments, there will be a certain lag in regulation, and it may be due to human negligence that the plant tissue culture effect makes an impact

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