Sea cucumber compound feed
A compound feed and sea cucumber technology, applied in the field of sea cucumber breeding, can solve the problems of affecting the survival and growth of sea cucumbers, increasing the cost of breeding, restricting the use of soybean meal, etc., and achieve the effects of promoting the growth of sea cucumbers, reducing the bait coefficient, and reducing the cost of feed
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[0019] Specific embodiments of the present invention will be described below.
[0020] 1. Preparation of Aspergillus niger spore suspension:
[0021] The preserved and rejuvenated Aspergillus niger fungus strain was inoculated on the PDA medium by punching, and cultured at 28°C for 3 days. After the hyphae covered the entire plate, the spores on the medium were washed off with sterile water. A suspension of Aspergillus niger spores (ie fungal seed liquid) is obtained.
[0022] Koji fermentation:
[0023] Take a certain amount of soybean meal, add water according to the ratio of material to water ratio of 80% and mix well, autoclave at 121°C for 20 minutes, and cool to room temperature. Put it into the fermentation container (sterile), insert the Aspergillus niger spore suspension (inoculation amount 10%v / w), put it in a 31℃ thermostat, and cultivate for 2 days. During the cultivation process, stir once at 24h and replenish with sterile water (replenish the lost water according to th...
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