Sea cucumber compound feed

A compound feed and sea cucumber technology, applied in the field of sea cucumber breeding, can solve the problems of affecting the survival and growth of sea cucumbers, increasing the cost of breeding, restricting the use of soybean meal, etc., and achieve the effects of promoting the growth of sea cucumbers, reducing the bait coefficient, and reducing the cost of feed

Inactive Publication Date: 2016-07-20
DALIAN HAIBAO FISHERY
9 Cites 8 Cited by

AI-Extracted Technical Summary

Problems solved by technology

However, at present, seaweed resources are limited and the price is high. If it is produced according to the original formula, it will increase the cost of cultivation
In the original formula, soybean meal is one of the sources of vegetable protein, and its proportion is not large. The main reason is...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Abstract

The present invention discloses a sea cucumber compound feed. The sea cucumber compound feed is characterized in that the compound feed is prepared from the following raw material ingredients in mass percentages: 16-40% of alga, 35-45% of sea mud, 2.7-27% of compound fermented soybean meal, 3-5% of fish meal, 3-5% of shell powder, 1-3% of yeast powder, 0.1-1% of multivitamin and 0.1-1% of polysaccharide. The sea cucumber compound feed is prepared by using the fermented soybean meal to replace a part of the alga, can the reduce farming costs, at the same time can also improve the quality of feed, and lower the feed conversion rates.

Application Domain

Technology Topic

Fish mealSea cucumber +8

Examples

  • Experimental program(1)

Example Embodiment

[0019] Specific embodiments of the present invention will be described below.
[0020] 1. Preparation of Aspergillus niger spore suspension:
[0021] The preserved and rejuvenated Aspergillus niger fungus strain was inoculated on the PDA medium by punching, and cultured at 28°C for 3 days. After the hyphae covered the entire plate, the spores on the medium were washed off with sterile water. A suspension of Aspergillus niger spores (ie fungal seed liquid) is obtained.
[0022] Koji fermentation:
[0023] Take a certain amount of soybean meal, add water according to the ratio of material to water ratio of 80% and mix well, autoclave at 121°C for 20 minutes, and cool to room temperature. Put it into the fermentation container (sterile), insert the Aspergillus niger spore suspension (inoculation amount 10%v/w), put it in a 31℃ thermostat, and cultivate for 2 days. During the cultivation process, stir once at 24h and replenish with sterile water (replenish the lost water according to the water content and maintain the water content), and stir twice without replenishing water on the second day. After the fermentation is completed, it is dried into koji at 50°C to make the weight the same as when the soybean meal is not fermented to obtain Aspergillus niger fermented soybean meal.
[0024] 2. Preparation of Trichoderma spore suspension:
[0025] The preserved and rejuvenated Trichoderma fungal strains were inoculated on the PDA medium by punching, and cultured at 28°C for 3 days. After the hyphae spread over the entire plate, rinse off the spores on the medium with sterile water. A suspension of Trichoderma spores (ie fungal seed liquid) is obtained.
[0026] Koji fermentation:
[0027] Take a certain amount of soybean meal, add water according to the ratio of material to water ratio of 80% and mix well, autoclave at 121°C for 20 minutes, and cool to room temperature. Put it into the fermentation container (sterile), insert the Trichoderma spore suspension (inoculation amount 10% v/w), put it in a 28°C thermostat, and incubate for 2 days. During the cultivation process, stir once at 24h and replenish with sterile water (replenish the lost water according to the water content and maintain the water content), and stir twice without replenishing water on the second day. After the fermentation is completed, it is dried into koji at 50°C to make the weight consistent with the unfermented soybean meal to obtain Trichoderma fermented soybean meal.
[0028] 3. Preparation of Shewanella suspension
[0029] Inoculate the conserved sea cucumber intestinal bacteria in 2216E liquid medium, culture at 28℃, 150rp/min shaker for about 12 hours, so that the number of bacteria reaches 10 10 cfu/mL, made into bacterial suspension.
[0030] Shewanella fermentation:
[0031] After mixing a certain amount of soybean meal at a material-to-water ratio of 80%, autoclave at 121°C for 20 minutes, cool to room temperature, put it into a fermentation container, and then access the above bacterial suspension (inoculation amount 1%v/w). ), put it in a 35°C thermostat and incubate for 3 days. During the first and second days of the cultivation process, the lost water was added according to the water content and the water content was maintained. On the third day, the water was not added and stirred twice to obtain the Shewanella fermented soybean meal. After the fermentation is completed, it is dried at 50°C to make the weight of the soybean meal consistent with the unfermented soybean meal.
[0032] Feeding test
[0033] The feeding experiment was conducted on the second floor of the Engineering Center of the Technical Department of Dalian Haibao Fishery Co., Ltd. A total of four test groups were set up, each with five repetitions, using 300L tanks, each tank containing 30 sea cucumber seedlings, weighing about 400g, and feeding every afternoon. The initial daily feeding amount was 3% of the body weight. Daily observation The bait situation determines and controls the amount of bait to ensure that there is no remaining bait, and the pool is poured according to the state of the sea cucumber.
[0034] This feeding experiment started on October 10, 2015 and ended on December 14, 2015. It lasted for 64 days, and the test water temperature was 14-19°C.
[0035] The Aspergillus niger fermented soybean meal, Trichoderma fermented soybean meal, and Shewanella fermented soybean meal that have been fermented by the above three bacteria are prepared into composite fermented soybean meal at a mass ratio of 16:16:68.
[0036] The CK feed formula of the control group was: seaweed 40.3%, sea mud 45%, soybean meal 2.7%, fish meal 5%, shell powder 4.8%, yeast powder 2%, multi-dimensional 0.1%, and polysaccharide 0.1%.
[0037] The feed formula of test group A is: seaweed 40.3%, sea mud 45%, compound fermented soybean meal 2.7%, fish meal 5%, shell meal 4.8%, yeast powder 2%, multi-dimensional 0.1%, polysaccharide 0.1%.
[0038] The feed formula of test group B is: seaweed 29.5%, sea mud 45%, compound fermented soybean meal 13.5%, fish meal 5%, shell meal 4.8%, yeast powder 2%, multi-dimensional 0.1%, polysaccharide 0.1%.
[0039] The feed formula of test group C is: 16% seaweed, 45% sea mud, 27% fermented soybean meal, 5% fish meal, 4.8% shell powder, 2% yeast powder, multi-dimensional 0.1%, and polysaccharide 0.1%.
[0040] Feeding sea cucumbers with various diets, the results are as follows:
[0041] Table 1 Effects of different feed formulas on the growth of sea cucumbers
[0042]
[0043] Note: * means significant difference compared with control (P<0.05)
[0044] It can be seen from the feeding results that the feed of test groups B and C can significantly promote the growth of sea cucumbers and reduce the feed coefficient.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

no PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Similar technology patents

Method for dry preservation of biological tissue

Owner:上海纽脉太惟医疗科技有限公司

Application of arsenic trioxide united ascorbic acid to treatment of colon cancer

InactiveCN110302211AEliminate side effectsTo achieve the purpose of "increasing efficiency and reducing toxicity"Organic active ingredientsInorganic active ingredientsToxicityINCREASED EFFECT
Owner:HARBIN MEDICAL UNIVERSITY

Classification and recommendation of technical efficacy words

  • Eliminate side effects
  • Reduce feed costs

Therapeutic prostatic thermotherapy

InactiveUS7089064B2Reducing treatmentEliminate side effectsMicrowave therapySurgical instruments for heatingProstate thermotherapyIn patient
Owner:BOSTON SCI SCIMED INC

c-MET MODULATORS AND METHOD OF USE

InactiveUS20070179130A1Strong specificityEliminate side effectsBiocideSenses disorderSignal transductionCellular activity
Owner:EXELIXIS INC

Gas-filled microbubbles and systems for gas delivery

InactiveUS20140010848A1Eliminate side effectsBiocideBreathing masksDipalmitoylphosphatidylcholinePhosphorylcholine
Owner:CHILDRENS MEDICAL CENT CORP

Meat duck feed and processing method

InactiveCN101991011AReduce feed costsAlleviate lack of protein resourcesFood processingAnimal feeding stuffFish mealAnimal science
Owner:HENAN MUHE GROUP FEED

Fragrant duck feed and feeding method

InactiveCN103053829AShort delivery timeReduce feed costsAnimal feeding stuffBroken riceLarge capacity
Owner:融水苗族自治县畜牧站

Breeding method for improving yield and quality of milk of cows in mountain areas of south China

InactiveCN102960298AIncrease milk production of dairy cowsReduce feed costsFood processingAnimal feeding stuffAnimal scienceCalcium magnesium phosphate
Owner:JIANGXI PROVINCE DAFU DAIRY IND GRP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products