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Novel method for emulsifying trace antigen

A new method and antigen technology, applied in the field of immunology, to achieve the effect of large-scale preparation and good application potential

Inactive Publication Date: 2016-07-20
张洪睿
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides a new method for microantigen emulsification, which is used to prepare water-in-oil immune mixture by mixing microantigen with Freund's adjuvant, etc., which solves the shortcomings of the existing antigen emulsification technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Preparation of Rabies Virus Immune Mixture

[0015] After mixing 0.2 ml of inactivated rabies virus purification at 1000 micrograms / ml with an equal volume of Freund's complete adjuvant in a 1.5 ml EP tube, add 6 steel balls with a diameter of 2 mm, seal the tube mouth, start the machine, and turn it on at 1500 rpm minutes, take it out after 1 minute, put it in an ice bath for 1 minute, and repeat 4 times. After the viral antigen and Freund's adjuvant were centrifuged at 1000g for 1 minute, the mixture did not appear to be stratified.

Embodiment 2

[0017] Preparation of Bovine Serum Albumin Immune Mixture

[0018] Dissolve 1 mg of bovine serum albumin in 1 ml of normal saline, add 0.1 ml to a 1.5 ml EP tube, add an equal volume of Freund's complete adjuvant, add 4 steel balls with a diameter of 2 mm, and follow the other steps in the examples 1. The emulsified mixture was not separated during centrifugation.

[0019] In the above examples, two types of particulate antigen and protein antigen are used, and the adjuvant used is Freund's complete adjuvant. Since the physical properties of Freund's incomplete adjuvant are the same as that of Freund's complete adjuvant, if selected Freund's incomplete adjuvant can also achieve the same emulsification effect. At the same time, the use of two types of antigens in the present invention shows that the present invention can be applied to macromolecular and small molecular antigens.

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Abstract

The invention provides a novel method for emulsifying a trace antigen.The antigen and a Freund's adjuvant at the same volume are mixed in a sterile 1.5ml EP tube, sterile steel balls are added in proportion, other EP tubes are used for balancing, a tube opening is sealed through a sealing film, a machine is started at the rotating speed of 1,500 revolutions per min, the tube is taken out 1 min later and placed in an ice bath for 1 min, the operation is repeated four times, 1,000 g of the emulsified antigen is centrifuged for 1 min, and the mixture is not layered.The novel method has the advantages that a trace tissue crusher is applied to emulsification of the trace antigen, in combination with the steel balls of different specifications, multiple different trace antigens can be effectively emulsified at the same time, the problems are effectively solved, large-scale preparation of the different antigens is completely achieved, and good application potential is achieved.

Description

technical field [0001] The invention belongs to the technical field of immunology, and provides a new method for emulsifying trace antigens, which is an improvement on the existing method for emulsifying trace antigens and immune adjuvants. Background technique [0002] Inducing a highly efficient immune response is the goal of most active immunizations based on antigen vaccination. In order to improve the immune effect of the antigen, the antigen is usually effectively mixed with an adjuvant. After the mixture is injected into the animal, the release time of the antigen can be prolonged to provide continuous antigen stimulation to obtain a good immune effect. Although the antigens used vary, Freund's adjuvant has always been the most commonly used immune adjuvant. Whether it is Freund's complete or incomplete adjuvant, due to the presence of a large amount of lanolin, its viscosity is extremely increased. When water-soluble antigens are added to this type of adjuvant, it i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F3/08B01F13/00
CPCB01F23/405B01F33/251
Inventor 张洪睿张宇
Owner 张洪睿