Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

New process and system for magnetic separation

A technology of a magnetic separation device and process, applied in the field of new processes and systems for magnetic separation, can solve the problems of inability to attract commercial interest and small capacity, and achieve the effect of increasing the binding capacity and being easy to implement

Active Publication Date: 2016-07-20
LAB ON A BEAD AB
View PDF8 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For large-scale separations, this volume is too small to be of commercial interest
However, porous magnetic particles (5m 2 / ml particles, see e.g. Protein Purification, Principles, High resolution Methods and Applications, J.C. Janson and L. Ryden, VCHPublicerslnc.1989, 40 pages) make it possible to develop alternatives to traditional chromatography techniques

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New process and system for magnetic separation
  • New process and system for magnetic separation
  • New process and system for magnetic separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1. Flowthrough processing (Flowthroughprocessing) - Separation of IgG by magnetic separation

[0085] Immunoglobulin G (IgG) is separated from larger sample volumes, eg, about 1 to 10,000 L, by utilizing magnetic particles and a magnetic separator device included in the flow system setup. The equipment includes a fermentor in which IgG is produced and to which magnetic particles having an affinity for IgG are added to capture the produced IgG, followed by various vessels for washing, elution and regeneration of the magnetic particles. The magnetic separator device is, for example, a magnetic filter where IgG-loaded magnetic particles are captured and accumulated from a large volume of cell culture medium. The magnetic separator device as disclosed herein can process cell culture medium and particles at a flow rate of 1 to 1000 L / min, which makes it possible to manipulate large volumes of cell culture medium in a short time.

[0086] The magnetic separator dev...

Embodiment 2

[0088] Example 2. Large-Scale Separation of Magnetic Particles in a Flow System Setup

[0089] Nearly 200 ml of settled magnetic particles were separated and accumulated within 35 minutes from a 10 LPBS solution using a magnetic separator device included in the flow system setup at a flow rate of 3 L / min.

[0090] Magnetic separator devices included in the flow system setup, including magnetic separator devices based on commercial devices (AutoMag Compact (AMC) from Eclipse Magnetics Ltd., Sheffield, UK). In the process described, the AMC original magnetic filter was modified with two additional outlets at the bottom of the housing, corresponding to the discharge valves, Figure 5 Component 3 in , thus allowing to simplify the discharge of the device and leading to efficient recovery and accumulation of magnetic particles, which are then recovered and recycled.

[0091] 10L cell culture / feed tank 1, waste container and 5L container for collecting magnetic particles as Fig...

Embodiment 3

[0100] Example 3. Binding and Elution of Immunoglobulins to Protein A

[0101] Magnetic particles were tested in large-scale cell culture experiments using the magnetic separator device included in the flow system setup. Scaled for 1000 grams or 10-1000 liters of cell culture, no special filtration will be performed to remove cells from the cell culture. The particles can be removed from the system after processing the cell culture batch, or the magnetic particles can be stored in the system in a bacteriostatic solution.

[0102] The magnetic separator unit included in the flow system setup was thoroughly rinsed with 10 liters of 1M NaOH solution. Circulate this solution through the system so that it contacts all pipes, valves and components. The contact time is about 30 minutes to efficiently clean and sanitize the filter.

[0103] The magnetic separator unit in the flow system setup, including all tubing and peristaltic pumps, was rinsed with deionized pyrogen-free wate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A process for large scale separation of molecules comprising the steps of providing magnetic porous particles having an affinity to said molecules to be separated; mixing said magnetic porous particles with a solution containing said molecules; bringing said mixture in contact with a magnetic separation device comprising a flow channel and at least one magnetic element; removing said at least one magnetic element and collecting the magnetic porous particles carrying said molecules; separating said molecules from said magnetic porous particles; obtaining a concentrated fraction of said molecules; and recirculating the magnetic porous particles .A system for performing this process.

Description

technical field [0001] The present description generally relates to novel processes, devices and systems for the separation of molecules and cells, in which magnetic filters and magnetic particles with high capacity and / or affinity for said molecules are the main components. Such processes and corresponding devices and systems are useful in chemical and biochemical processes in which one or more reactants, such as catalysts or enzymes, are bound to a solid medium, such as in chemical and biochemical synthesis and in various purifications step. [0002] The process, apparatus and system are also applicable to the removal and / or enrichment of desired or unwanted components, such as, but not limited to, the removal of pharmaceutical residues, heavy metals or other unwanted contaminants from drinking water, and the enrichment of chemical and intermediate and final products in biochemical synthesis, production and recovery. Background technique [0003] Techniques for the separ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/281B01D15/38C08G81/00
CPCB01J20/289B01J20/3204B01J20/321B01J20/3212B01J20/3219B01J20/3274B01D15/3885B01J20/28009B01J20/282
Inventor 斯文·奥斯卡尔森克里斯托弗·埃里克松佩尔·奥洛夫·埃里克松
Owner LAB ON A BEAD AB
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com