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Use of cinchonine in preparation of anti-tumor drugs

An anti-tumor drug, the technology of cinchonine, which is applied in the direction of anti-tumor drugs, drug combinations, and resistance to vector-borne diseases, can solve the problem that cinchonine has not yet inhibited tumor cell growth, and achieves inhibition of tumor cell growth, small side effects, and toxic side effects small effect

Inactive Publication Date: 2016-07-27
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report that cinchonine inhibits the growth of tumor cells.

Method used

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  • Use of cinchonine in preparation of anti-tumor drugs
  • Use of cinchonine in preparation of anti-tumor drugs
  • Use of cinchonine in preparation of anti-tumor drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0016] 1. Effect of cinchonine on tumor cell growth:

[0017] Hela cells and A-549 cells (purchased from Shenyang Wanlei Biotechnology Co., Ltd.) were cultured, seeded into 96-well plates at a cell density of 4000 cells / well, and after culturing for 24 hours (h), 1×10 -6 , 5×10 -6 , 1×10 -5 , 2.5×10 -5 , 5×10 -5 , 1×10 -4 , 2.5×10 -4 , 5×10 -4 The cinchonine of mol / L concentration, measure cell growth state with MTT method after 72h and 96h respectively, its result sees figure 1 , showing a concentration-dependent inhibition of the growth of Hela cells, figure 2 It was shown that cinchonine inhibited the growth of A-549 cells in a concentration-dependent manner.

[0018] MTT method: Add 20 μl of 5 mg / ml MTT solution to each well, take it out after incubating for 3-4 hours in the incubator, discard the supernatant, add 150 μl dimethyl sulfoxide to each well to dissolve the formazan precipitate, and use a microplate reader at 490 nm Measure the absorbance value below. ...

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Abstract

The invention discloses a use of cinchonine in preparation of anti-tumor drugs. Cinchonine is found out to have a role in inhibiting growth of tumor cells during screening of a large number of anti-tumor drugs. When cinchonine is clinically applied in treatment of malaria, generated side effects are smaller than side effects generated by conventional anti-tumor drugs, and cinchonine is the new anti-tumor drug having small side effects.

Description

technical field [0001] The present invention relates to a new application of cinchonine. Background technique [0002] In recent years, the incidence of tumors has been increasing day by day, and new and more effective anti-tumor drugs need to be developed. [0003] Cinchonine ((1S)-quinolin-4-yl(5-vinylquinine-2-yl)methanol) is a quinoline-type alkaloid, a kind of cinchona alkaloid, also known as cinchona, weak Cinchonidine is a stereoisomer of cinchonidine. Molecular formula C19H22N2O. Molecular weight 294.40. White needle-like or prismatic crystals can be obtained from ether. Melting point 58~60℃, [α]D15+48°(ethanol, C=1). Sublimation begins at 220°C. Soluble in alcohol, ether, acetone, benzene and chloroform, almost insoluble in cold water. The color becomes darker under the light, and its aqueous solution is reacted with phosphoric acid and organic acid (especially acetic acid) at 98-100°C, and most or almost all of them are converted into cineretoxin, which does...

Claims

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Application Information

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IPC IPC(8): A61K31/49A61P35/00
CPCY02A50/30
Inventor 周立军祁永浩
Owner TIANJIN UNIV
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