Electrical impedance method for measuring soluble sugar content in catalpa bungei stem leaf and cold resistance of catalpa bungei

A measurement method, electrical impedance technology, applied in the direction of material impedance, etc., can solve the problems of cumbersome, destructive hysteresis, and cannot meet the needs of fast and real-time production

Inactive Publication Date: 2016-07-27
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the traditional methods for the determination of plant soluble sugars mainly include the anthrone method and the phenol method, but the traditional methods are not only cumbersome, laborious, time-consuming, but also destructive and hysteresis, and cannot meet the rapid and real-time requirements in production.

Method used

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  • Electrical impedance method for measuring soluble sugar content in catalpa bungei stem leaf and cold resistance of catalpa bungei
  • Electrical impedance method for measuring soluble sugar content in catalpa bungei stem leaf and cold resistance of catalpa bungei
  • Electrical impedance method for measuring soluble sugar content in catalpa bungei stem leaf and cold resistance of catalpa bungei

Examples

Experimental program
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Effect test

Embodiment 1

[0108] After the stems were washed and dried with deionized water, they were packed into plastic bags respectively, repeated 8 times, and a small amount of deionized water was sprayed into the bags to prevent the material from being too cold. Seven temperature gradients (see Table 1) were set for the freezing treatment temperature, with 4°C as the control, which included the temperature at which all samples survived and all died. The cooling rate is 6℃·h -1 , kept at the processing temperature for 4 hours, then thawed at 4°C for 24 hours, and then tested.

[0109] Table 1 In Example 1 of the present invention, the determination of the cold resistance of Catalpa spp.

[0110]

[0111] Randomly select 8 sections of 15mm long stems after thawing for electrical impedance measurement:

[0112] Impedance meter (HP4284A, USA) was used for EIS measurement, Ag / AgCl electrode was used in the test, electrode gel was added to the electrode, and the cross-section of the sample was con...

Embodiment 2

[0115] After the leaves were washed and dried with deionized water, they were packed into plastic bags, repeated 8 times, and a small amount of deionized water was sprayed into the bags to prevent the material from being too cold. Seven temperature gradients (Table 2) were set for the freezing treatment temperature, with 4°C as the control, which included the temperature at which all samples survived and all died. The cooling rate is 6℃·h -1 , kept at the processing temperature for 4 hours, then thawed at 4°C for 24 hours, and then tested.

[0116] Table 2 In the embodiment of the present invention 2, the determination of freezing treatment setting temperature of the cold resistance of the leaves of Catalpa catalpa

[0117]

[0118] Randomly select 8 rectangular leaf segments without veins after thawing, the size is 4mm×10mm, and conduct electrical impedance measurement:

[0119] Impedance meter (HP4284A, USA) was used for EIS measurement, Ag / AgCl electrode was used in th...

Embodiment 3

[0122] After washing and drying the stems of Chinese catalpa with deionized water, put them in an oven at 80°C and dry them for 2 days. After taking them out, use the anthrone colorimetric method to measure the content of soluble sugar in them;

[0123] According to the scheme measurement of embodiment 1, 75 groups of samples of Chinese catalpa stem are measured for electrical impedance, obtain the electrical impedance value of 75 groups of golden catalpa stem samples, then with reference to Meng Yu etc. (Meng Yu, Di Bao, Zhang Gang, Feng Feng Xinguo, Xu Jiancheng, Tian Jun. Correlation analysis between soluble sugar and starch content and electrical impedance of birch roots under waterlogging stress. Acta Biophysica Sinica, 2013, 29(6):450~460.) Determination of EIS parameters , using LEVM8.06 software, using the single-DCE model for data fitting, and finally obtained the high frequency resistivity r (Ωm), low frequency resistivity r 1 (Ωm), extracellular resistivity r e (Ω...

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Abstract

The present invention provides an electrical impedance method for measuring soluble sugar content in catalpa bungei stem leaf. The method comprises the steps of: detecting an electrical impedance spectroscopy of a catalpa bungei stem leaf sample to be tested; according to the electrical impedance spectroscopy of the sample to be tested, determining an appropriate equivalent circuit; according to the equivalent circuit, fitting the data of the electrical impedance spectroscopy of the sample to be tested to obtain electrical impedance parameters of the sample to be tested; and according to a regression model of the predetermined electrical impedance parameters of the catalpa bungei stem leaf with soluble sugar content, and the electrical impedance parameters of the sample to be tested, acquiring the soluble sugar content of the catalpa bungei stem leaf. The method provided by the invention establishes a regression model of the electrical impedance parameters and soluble sugar content of the catalpa bungei stem leaf, thus calculating the soluble sugar content of the catalpa bungei stem leaf based on the measured impedance parameters. The method can realize fast real-time diagnosis of the soluble sugar content and can obtain the cold resistance of the catalpa bungei stem leaf through the Logistic equation.

Description

technical field [0001] The invention relates to the technical field of plant component content detection, in particular to a method for measuring the soluble sugar content of catalpa stems and leaves and the cold resistance of catalpa by electrical impedance method. Background technique [0002] The adaptation of plants to low temperature stress is closely related to their own physiological and biochemical activities such as cell membranes, protective enzyme systems, and osmotic adjustment substances. Jian Lingcheng et al. believe that plants mainly rely on soluble sugars for osmotic adjustment at the initial stage of low temperature stress, and when the stress is further deepened, other osmotic substances such as soluble proteins play a role. Therefore, the content of soluble sugars is often used as a physical and chemical indicator for identifying plant cold resistance. Timely and accurate detection and diagnosis of changes in soluble sugar content in plants is helpful to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/02
Inventor 张钢邸葆钱稷孟昱刘扬向地英弓瑞娟邢宝武东霞
Owner HEBEI AGRICULTURAL UNIV.
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