A kind of preparation method of insulin derivative

Abstract

The present invention aims at the problem of low yield in the isoelectric precipitation method used in the preparation process of existing insulin derivatives, and provides a preparation method of insulin derivatives. After the reaction is completed, divalent or trivalent metal ions are added to the reaction solution, so that the crude product of insulin derivatives or insulin derivative precursors can be completely precipitated from the reaction solution, and the product loss remaining in the supernatant It can be reduced to less than 7% of the control example (without adding metal ions), and some can even be reduced to less than 1% or undetectable, greatly improving the product yield.

Description

technical field

[0001] The invention relates to a preparation method of insulin derivatives. Background technique

[0002] Diabetes is a common endocrine and metabolic disease. In recent years, the prevalence of diabetes in the world has been increasing. In China, with the change of people's lifestyle and the acceleration of the aging process, the prevalence of diabetes is increasing rapidly. At present, the number of diabetic patients in China has exceeded 1.14 billions, bringing heavy burdens to individuals, families and society.

[0003] Diabetic patients generally have relative or absolute insufficient insulin secretion, so insulin therapy has been regarded as an important means to treat diabetes and make blood sugar well controlled. In the 1970s, people developed recombinant human insulin through genetic engineering technology. Later, in order to obtain drugs with different action time curves such as quick-acting or long-acting, people successively developed a series...

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