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A fungal remediation method for heavily chromium-contaminated soil

A technology of chromium-contaminated soil and remediation method, applied in the restoration of polluted soil, etc., can solve problems such as high chromium content, retention, and difficulty in practical application, and achieve simple operation and environment-friendly effects

Active Publication Date: 2019-05-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But most of them stay in the stage of laboratory research, and it is difficult to carry out practical application
The main reason is that the soil in chromium-contaminated sites often has the characteristics of high salinity and high chromium content, which seriously inhibits the growth, metabolism and reduction ability of microorganisms. Therefore, how to use microbial technology to repair heavily chromium-contaminated soil has become a current research Applied Difficulties

Method used

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  • A fungal remediation method for heavily chromium-contaminated soil
  • A fungal remediation method for heavily chromium-contaminated soil
  • A fungal remediation method for heavily chromium-contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: A kind of fungal remediation method of heavily chromium-contaminated soil

[0028] (1) production Penicillium oxalicum SL2 spore suspension. Will Penicillium oxalicum SL2 was cultured on a PDA medium plate for 10 days, the spores on the surface of the plate were washed with water, and the spores were obtained after shaking and dispersing Penicillium oxalicum Spore suspension of SL2. Penicillium oxalicum SL2 was screened by our research group and belongs to the prior art. It was preserved in the China Type Culture Collection and Management Center with the preservation number CCTCC NO: M2014505, October 22, 2014.

[0029] (2) The heavily chromium-contaminated soil was collected from a decommissioned chemical factory in Hangzhou. The soil is alkaline soil (pH 10.2), the soil organic matter content is low (5.16g / kg), and the soil cation exchange capacity is 61.97cmol / kg , the total chromium in the soil is 7069.6mg / kg, and the six price is 4385.7mg / ...

Embodiment 2

[0035] Example 2: pH pair Penicillium oxalicum Effect of SL2 Removal of Cr(Ⅵ)

[0036] Prepare 5g / L Cr(VI) solution: weigh 7.5g potassium dichromate and bake at 110°C for 2h, then weigh 7.073g and dissolve in 500mL water to make 5g / L Cr(VI) solution. Filter the 5g / L Cr(VI) solution with a 0.22um sterile filter head to adjust the Cr(VI) concentration in the medium.

[0037]Preparation of potato medium: take fresh potatoes, peel them, dig out the bud eyes, wash them, and slice them. Weigh 200g, cut into small pieces, add 1000ml of water to boil for 30min, filter with double gauze, and make up the filtrate to 1000ml of water. Add 10 g of glucose to the filtrate, dissolve it and cool it down, distribute 50 ml each into 250 ml Erlenmeyer flasks, and sterilize at 115°C for 20 minutes.

[0038] Use a sterile Cr(VI) solution to adjust the Cr(VI) concentration in the medium so that the Cr(VI) in the system is about 160 mg / L, and adjust the pH to 3, 4, 5, 6, 7, 8 with concentrated ...

Embodiment 3

[0039] Embodiment 3: inoculum size pair Penicillium oxalicum Effect of SL2 Removal of Cr(Ⅵ)

[0040] The preparation concentration is 10 8 individual / L spore suspension, and then use the gradient dilution method to obtain a concentration of 10 7 pcs / L, 10 6 pcs / L, 10 5 / L of spore suspension. Add sterile Cr(Ⅵ) solution to the Erlenmeyer flask filled with potato medium, so that the initial concentration of Cr(Ⅵ) in the system is about 160mg L -1 . The prepared spore suspension was inoculated at a ratio of 1:100 (V / V), treated with sterile water as a control, cultured with shaking at 30°C and 200 rpm, and samples were taken at different times to determine the concentration of Cr(Ⅵ) in the culture solution . The result is as Figure 5 Shown: On the third day of culture, the higher the concentration of inoculated spores, the lower the concentration of Cr(Ⅵ) in the system, but the Cr(Ⅵ) in all treatment groups can be reduced on the fifth day. show Penicillium oxalicum ...

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Abstract

The invention discloses a fungus repair method for severe chromium-contaminated soil. According to the method, spores of Penicillium oxalicum SL2 are taken as a repair bacterial strain source, severe chromium-contaminated soil is mixed with a potato culture medium according to proportion to prepare a slurry suitable for germination and growth of bacterial strain spores, and a certain amount of oxalic acid is added to accelerate the repair efficiency of chromium contamination of the soil. The slurry inoculated with spores of Penicillium oxalicum SL2 is cultured in an environment of 30 DEG C, and after 10 days, the concentration of Cr (VI) in a soil leaching liquor is reduced to 0.7mg / L from 231.8mg / L, the removal rate reaches 99.7%, and the concentration of bioavailable form Cr (VI) in the repaired soil is reduced to 44.3mg / kg, which is reduced by 98.1% in comparison with soil treated by using a common clear water leaching method. The method disclosed by the invention is simple in operation, is environment-friendly, and can provide technical support for microbial remediation of severe chromium-contaminated soil.

Description

technical field [0001] The invention relates to a fungal restoration method for heavily chromium-contaminated soil, and belongs to the technical field of microbial application. Background technique [0002] my country is the country that produces the most chromium salts. The extensive production process and the untimely implementation of the chromium removal process have resulted in a large number of chromium-contaminated sites. According to statistics, more than 100 places in my country are polluted by chromium, affecting an area of ​​more than 50,000 square kilometers, and the pollution level of some polluted sites exceeds the standard by 100 times, which seriously threatens my country's ecological security and people's health. Therefore, the rapid and effective restoration of chromium-contaminated soil has imminent. [0003] After entering the soil, chromium mainly exists in two stable forms, Cr(Ⅲ) and Cr(Ⅵ). Among them, Cr(Ⅵ) is teratogenic, carcinogenic and mutagenic, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10
CPCB09C1/105
Inventor 施积炎叶斌晖龙碧波张舒叶基恩邹丽娜刘青林
Owner ZHEJIANG UNIV