Production method of calcium pyruvate beverage
A calcium pyruvate and beverage technology, applied in fermentation, food science, etc., can solve the problems of increasing the production cost of calcium pyruvate products, difficulty in meeting the national environmental requirements, and the safety of pyruvate to be considered, so as to inhibit free radicals The generation of high-purity products and the effect of enhancing human endurance
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Embodiment 1
[0016] Calcium pyruvate drink is prepared by the following process steps in the present embodiment:
[0017] (1) Fermentation medium ingredients:
[0018] At room temperature, put 90g of oral glucose, 3g of food-grade peptone, and 30g of food-grade calcium carbonate in 1000mL of distilled water, mix the above raw materials, the pH of the mixture is 5.0, and sterilize the above-mentioned mixture at 108°C for 10 minutes. After sterilizing, put it into the fermenter for standby;
[0019] (2) Fermentation strain culture:
[0020] Cultivate the strains of Toructus candida in shake flasks at 30°C for 16 hours. When the maximum growth logarithmic phase of the strains is reached, enter the seed tank for seed expansion cultivation, and the strains are cultivated in the seed tanks for 20 hours. When the strain concentration reaches 0.8%, transfer to the above-mentioned fermentation tank for fermentation;
[0021] (3) Fermentation:
[0022] During the fermentation process, the temper...
Embodiment 2
[0030] Calcium pyruvate drink is prepared by the following process steps in the present embodiment:
[0031] (1) Fermentation medium ingredients:
[0032] At room temperature, put 95g of glucose, 7g of peptone, and 33g of calcium carbonate into 1000mL of distilled water, mix the above mixture well, the pH value of the mixture is 5.1, and sterilize the above mixture at 110°C for 11 minutes, then put into the fermenter for subsequent use;
[0033] (2) Fermentation strain culture:
[0034] Cultivate the strains of Torulopsis candida in shake flasks at 30.5°C for 18 hours. When the maximum growth logarithmic phase of the strains is reached, enter the seed tank for seed expansion cultivation, and the strains are cultivated in the seed tanks for 21 hours. When the strain concentration reaches 0.9%, transfer to the above-mentioned fermentation tank for fermentation and cultivation;
[0035] (3) Fermentation:
[0036] During the fermentation process, the temperature is controlled ...
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