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A method for studying the regulatory effect of purα protein on bace1 gene expression

A technology of gene expression and α protein, which is applied in the field of studying the regulation effect of Purα protein on BACE1 gene expression, which can solve problems such as unreported

Inactive Publication Date: 2019-09-03
NINGXIA MEDICAL UNIV +1
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no report on the regulation of Purα protein on the expression of BACE1 gene.

Method used

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  • A method for studying the regulatory effect of purα protein on bace1 gene expression
  • A method for studying the regulatory effect of purα protein on bace1 gene expression
  • A method for studying the regulatory effect of purα protein on bace1 gene expression

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Embodiment 1

[0026] A method for studying the regulatory effect of Purα protein on BACE1 gene expression, the method includes three aspects, and the three aspects are respectively used to determine the influence of Purα protein on BACE1 gene promoter activity and the influence on BACE1 gene expression And the effect on the translation level of BACE1 protein.

[0027] In the three aspects, the first aspect is specifically to determine the active influence of the Purα protein on the BACE1 gene promoter through a reporter gene, and the second aspect is specifically to transfect the eukaryotic expression vector of the Purα protein into the HT22 cell line for transcription The effect of Purα protein on the expression of BACE1 gene is detected horizontally, and the third aspect is specifically to detect the effect of Purα protein on the translation level of BACE1 protein by western blotting.

[0028] The 5-terminal non-coding region of the BACE1 promoter has multiple GC-rich regions, and this re...

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Abstract

The invention mainly belongs to the technical field of regulation of gene expression and particularly relates to a method for studying a regulating effect of Pur alpha protein on BACE1 gene expression. The method comprises three aspects as follows: firstly, the effect of the Pur alpha protein on activity of a promoter of the BACE1 gene is determined through a reporter gene; secondly, an eukaryotic expression vector of the Pur alpha protein is transfected into an HT22 cell line, and the effect of the Pur alpha protein on BACE1 gene expression is detected at the RNA transcriptional level with a QPCR (quantitative polymerase chain reaction) technology; thirdly, the effect of the Pur alpha protein on the translation level of the Pur alpha protein on BACE1 protein is detected with a Western blotting method. The invention provides the method for studying the regulating effect of the Pur alpha protein on BACE1 gene expression.

Description

technical field [0001] The invention mainly belongs to the technical field of gene expression regulation, and specifically relates to a method for studying the regulation effect of Purα protein on BACE1 gene expression. Background technique [0002] Alzheimer's disease (AD), also known as senile dementia, is a degenerative disease of the central nervous system and the most common type of senile dementia. The main manifestations are neuropsychiatric symptoms such as progressive memory impairment, cognitive dysfunction, personality changes, and language barriers, which seriously affect social, occupational, and life functions. [0003] At present, the basic etiology of AD is still not completely understood, but a large number of data confirm that it is related to the generation and accumulation of β-amyloid protein (amyloid β peptide, Aβ), which leads to the toxic damage to nerve cells. β-amyloid is formed through the activity of several enzymes, one of which is the β-site am...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6897C12Q1/6851G01N33/68G01N33/573
Inventor 袁程敏
Owner NINGXIA MEDICAL UNIV