Unlock instant, AI-driven research and patent intelligence for your innovation.

Lead ion direct electrochemical detection method for probe-integrated functional nucleic acid modified electrode

A technology for modifying electrodes and functional nucleic acids, which can be used in electrochemical variables of materials, measuring devices, scientific instruments, etc., can solve problems such as inapplicability, and achieve the effects of strong functionality, low energy consumption, and low cost

Inactive Publication Date: 2016-08-24
NANTONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF THE PEOPLES REPUBLIC OF CHINA
View PDF9 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since fluorescence detection, ultraviolet detection and electrochemiluminescence detection all require relatively large and expensive detection instruments, these methods are not suitable for some relatively simple workplaces and field operations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lead ion direct electrochemical detection method for probe-integrated functional nucleic acid modified electrode
  • Lead ion direct electrochemical detection method for probe-integrated functional nucleic acid modified electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] (1) Preparation of probe-integrated functional nucleic acid-modified electrodes

[0019] The gold nanoparticle-modified electrode was self-assembled in a buffer solution containing 1 μM thiol-modified 17E DNAzyme for 12 hours; the buffer solution was a 20 mM Tris-HCl solution containing 140 mM NaCl and 5 mM KCl at pH 7.5; Take out the electrode and wash it with the buffer solution, then put the electrode into the above buffer solution with 1mM mercaptohexanol for passivation for 2 hours, wash with the same buffer solution to remove unassembled mercaptoethanol; finally transfer the electrode to the buffer containing The 1 μM substrate 17DS solution was assembled for 12 hours; the 3' end of the substrate 17DS was modified with a ferrocene probe molecule; and the electrode was washed three times with the above buffer solution to obtain a probe-integrated functional nucleic acid modified electrode.

[0020] (2) Detection of lead ions in lake water samples

[0021] The prep...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a lead ion direct electrochemical detection method for a probe-integrated functional nucleic acid modified electrode. The probe-integrated functional nucleic acid modified electrode is immersed in a buffering solution of to-be-detected lead ions for 20-30 min, the electrode is taken out, washed and subjected to DPV determination in a 0.1 M NaClO4 electrolyte solution, and the lead ion concentration in a sample is calculated according to the change rate of DPV peak current signals scanned and recorded in the range of 0.1-0.6 V. The method is good in selectivity, sensitive, simple and quick.

Description

technical field [0001] The invention relates to a method for directly electrochemically detecting lead ions with a probe-integrated functional nucleic acid modified electrode. Background technique [0002] Lead ion is a common harmful heavy metal ion, and its monitoring / detection is of great significance to human health and environmental monitoring. In the past few decades, people have developed various methods such as atomic absorption / emission spectroscopy, fluorescence spectroscopy, mass spectrometry, photonic crystals, etc. to monitor / detect lead ions. In recent years, the research team of Lu Yi in the United States obtained the functional nucleic acid 17E DNAzyme sensitive to lead ions through in vitro screening technology. They used 17E DNAzyme as the lead ion recognition element and gold nanoparticles as the sensing element to develop a colorimetric sensor to detect lead. ions (Liu, J.; Lu, Y., Nature Protocols 2006, 1, 246-252.). Wang’s group developed a label-free...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/26G01N27/333G01N27/327G01N27/48
CPCG01N27/26G01N27/327G01N27/3275G01N27/333G01N27/48
Inventor 洪华张爱平施筱迪王海天洪昱王红卫王超颖申晓萍
Owner NANTONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF THE PEOPLES REPUBLIC OF CHINA